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乙型肝炎病毒X区基因变异与原发性肝癌的关系研究 被引量：2

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摘要 目的初步研究乙型肝炎病毒(HBV)X区基因变异与原发性肝癌的关系。方法收集39例HBV感染患者的肝(癌)石蜡组织标本,其中慢性乙型肝炎(CHB)14例、肝细胞癌(HCC)25例。采用聚合酶链式反应(PCR)方法扩增组织中HBV X区基因,并对PCR产物进行DNA测序,分析常见变异位点的变异情况。结果 1.HCC组与CHB组相比,在X区发生插入/缺失变异,联合变异的位点及例数增多,并且A1762T与G1764A常发生双突变。2.HBV X区变异频率较高位点依次是C1655T/G、A1605C/G、A1762T、G1764A、A1772B、A1645C、C1687A、G1776T。结论 HBV X区存在点突变、插入/缺失变异及联合变异,可能在HCC的发生和发展过程中起重要作用。
作者 杨伟 李增彩 倪秀莹 王涛
机构地区 山东省潍坊市第八九医院传染科
出处 《肝脏》 2016年第1期45-48,共4页 Chinese Hepatology
关键词 原发性肝癌 HBV X基因 变异
分类号 R512.62 [医药卫生—内科学] R735.7 [医药卫生—肿瘤]
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参考文献7

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二级参考文献22

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肝脏
2016年 第1期

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