结直肠癌患者血清miRNA-200b、miRNA-200c水平表达及其与临床特征的关系研究

The expression of serum miRNA-200b and miRNA-200c and the correlation with clinical features in colorectal cancer patients

目的探讨结直肠癌患者血清中miRNA（miR）-200b和miR-200c的表达及其与患者临床病理特征及预后的关系。方法采用实时定量聚合酶联反应（PCR）方法检测90例结直肠癌患者（观察组）术前血清标本和50例健康志愿者（对照组）血液样品中miR-200b和miR-200c的表达水平，并结合患者临床特征进行分析。结果观察组血清miR-200b和miR-200c的表达水平明显低于对照组。miR-200b和miR-200c的表达与肿瘤组织分化程度、TNM分期、淋巴结转移密切相关；miR-200b或miR-200c低表达与不良预后有关（P〈0．01）。结论结直肠癌患者血清中miR-200b和miR-200c的表达与组织学分化、临床分期、淋巴结转移有关。血清miR-200b和miR-200c的检测有可能作为结直肠癌诊断及判断预后的生物标志。

Objective To explore the expression of serum miR-200b and miR-200c and the correlation with clinical features in colorectal cancer patients. Methods 90 colorectal cancer patients （observation group） and 50 healthy volunteers（ control group） were detected to explore the expression of miR-200b and miR-200c by real-time quantitative polymerase chain reaction （qRT-PCR）, and the relationship between miR-200b and miR-200c and clinical features was analyzed. Results The expression levels of serum miR-200b and miR-200c in observation group were significant lower than that in the control group. Relationships between expression of serum miR-200b and miR-200c and clinic features were observed in histological differentiation, TNM stage,lymph node metastasis. Lower expression of miR-200b and miR-200c had poorer prognosis in colorectal cancer patients（P 〈 0.01 ）. Conclusion The expression of serum miR-200b and miR-200c in patients with colorectal cancer had related to histo- logical differentiation ,TNM stage,lymph node metastasis. The detection of serum miR-200b and miR-200c might be used as the biomarkers of early diagnosis and estimating prognosis of colorectal cancer.