参芪益智散挥发性成分分析

Analysis of Volatile Components in Compound Shengqiyizhi Powder

目的:分析参芪益智散的挥发性成分。方法:顶空固相微萃取法(HS-SPME)结合气质联用技术(GC-MS)分析复方粉末中的挥发性成分;水蒸气蒸馏法(SD)结合GC-MS对复方挥发油进行分析鉴定。结果:HS-SPME-GC-MS法从复方混合物粉末中检测出挥发性成分117种,鉴定出70种,相对含量占总挥发性成分的94.46%,其中含量最高的为β-雪松烯(19.31%),其次为衣兰烯(15.56%)、β-细辛醚(7.36%);SD-GC-MS法从复方挥发油成分中检测出66种,鉴定出43种,相对含量占总挥发性成分的77.41%,其中含量最高的为β-细辛醚(16.73%),其次为丁香酚甲醚(6.74%)、衣兰烯(5.81%)。结论:参芪益智散挥发性成分主要为β-细辛醚、α-细辛醚、β-雪松烯、衣兰烯、榄香素、芳樟醇等。本研究为参芪益智散挥发性成分分析提供了方法学基础。

Objective： To analyze the volatile components in compound Shenqiyizhi powder. Methods： The technique of headspace solid phase micro extraction（ HS-SPME） with gas chromatography-mass spectrometry（ GC-MS） was applied to study the volatile components in the compound powder. The compound essential oil was analyzed by steam distillation（ SD） with GC-MS. Results： A total of 117 kinds of volatile components were detected,and among them,70 kinds of chemical compositions were identified from the compound powder,accounting for 94. 46% of the total volatile components. The component with the highest content was β-himachalene followed by ylangene（15. 56%） and β-asarone（7. 36%）. Totally 66 kinds of volatile components were detected from the compound essential oil by SD-GC-MS,and among them,43 kinds were identified,accounting for 77. 41% of the total volatile components. The results indicated that the main volatile components were β-asarone（ 16. 73%）,eugenol methyl ether（ 6. 74%） and ylangene（5. 81%）. Conclusion： The main volatile components in Shenqiyizhi powders are β-cedrene,ylangene,β-asarone,α-asarone and elemicin etc. The research can provide scientific basis for the analysis of volatile components.