自体黑色素细胞培植疗法治疗白癜风及其机制研究

The treatment of vitiligo by autologous melanocyte implantation

目的 探讨自体黑色素细胞培植疗法对于治疗白癜风的临床效果.方法 选取我院2013年2月到2014年2月期间收治63例患者为研究对象,对这些患者主要采用自体黑色素细胞培植疗法,3个月后对所有患者的康复情况进行总结.蛋白质印迹法（Western blot）检测治疗前后病变皮肤XRCC1和HSP-90表达变化.结果 通过自体黑色素细胞培植疗法治疗以后,38例（60.3％）患者痊愈,10例（15.9％）患者病情明显好转,需要继续观察,15例（23.8％）患者病情无明显变化甚至有所恶化,需配合其他方法继续治疗.和对照组相比,治疗前XRCC1表达水平显著增加,而HSP-90表达水平降低;治疗后XRCC1表达水平降低,而HSP-90表达水平略升高.结论 自体黑色素细胞培植疗法治疗白癜风具有较好的疗效,其治疗效果与XRCC1和HSP-90密切相关.

Objective To investigate the clinical effect of autologous melanoma cell implantation on vitiligo.Methods From February 2013 to February 2014,63 patients were treated by autologous melanocyte implantation.The rehabilitation of all patients were summarized three months after the treatment.Western blot was applied to detect the expression of XRCC1 and HSP-90.Results After implantation of autologous melanocyte,38 cases （60.3%） were cured,and the condition of 10 cases （15.9%） patients was improved markedly.There was no obvious changes in 15 （23.8%） patients.Compared with control,the expression of SRCC1 was significantly increased,while HSP-90 expression was decreased before treatment.The expression of SRCC1 was decreased,while HSP-90 expression was slightly increased after treatment.Conclusion Autologous melanocyte implantation has good curative effect on vitiligo.This effect is associated with XRCC1 and HSP-90 expression.