重庆市万州区在园儿童手足口病病原携带情况调查

Investigation on the carrying status of hand-foot-mouth disease pathogens among children in kindergartens in Wanzhou district of Chongqing

目的了解重庆市万州区在园儿童手足口病病原携带情况,为分析手足口病发病规律、科学防控提供参考依据。方法采用随机抽样的方式,采集不同季节、城市与农村健康在园儿童肛拭子标本,以荧光RT-PCR法检测手足口病肠道病毒通用型（EV）、肠道病毒71型（EV71）、柯萨奇病毒CA16、CA6、CA10型。结果 1 276份标本检出EV核酸阳性211份,阳性率为16.54%。其中EV71、CA6、CA16、CA10及HEV分别占13.74%、6.16%、4.74%、1.90%、73.46%。手足口病病毒携带率流行期高于非流行期,差异有统计学意义（χ-2=14.97,P〈0.05）,农村高于城市,差异有统计学意义（χ-2=9.184,P〈0.05）,男、女差异无统计学意义（χ-2=0.004 2,P=0.948 2）,各年龄组差异有统计学意义（χ-2=36.26,P〈0.05）。结论重庆市万州区在园儿童手足口病病原携带率较高,不同区域、季节、年龄组差异有统计学意义,应采取有针对性的防控策略。

Objective To investigate the carrying status of pathogens of hand- foot- mouth disease（ HFMD） among children in kindergartens in Wanzhou district of Chongqing,so as to provide references for identifying the pattern of this disease and scientifically preventing and controlling the disease. Methods Anal swabs were randomly collected from healthy children in kindergartens in both urban and rural areas in different seasons,and the enterovirus（ EV）,enterovirus 71（ EV71）,and CA6,CA10,and CA16 were detected by fluorescent PCR. Results Of the total 1 276 samples collected,211 presented positive EV nucleic with the positive rate of 16. 54%. EV71,CA6,CA16,CA10 and HEV accounted for 13. 74%,6. 16%,4. 74%,1. 90%,73. 46%,respectively. The carrying rate of viruses of HFMD in epidemic season was higher than in non- epidemic season,with the differences statistically significant（ χ-2= 14. 97,P〈0. 05）,and higher in rural area than in urban area,with the differences statistically significant（ χ-2= 9. 184,P〈0. 05）. There were statistical significance on the differences between different age groups（ χ-2= 36. 26,P〈0. 05）,while no statistical significance on the differences between males and females（ χ-2= 0. 004 2,P = 0. 948 2）. Conclusion The pathogens carrier rates were relatively high and the difference among different areas,epidemic seasons and ages were obvious in Wanzhou district of Chongqing. Therefore,targeted prevention and control is needed for this disease.