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24小时培养法与PCR扩增tcd B基因法鉴定艰难梭菌的比较

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摘要 目的分析比较24 h培养法与PCR扩增tcd B基因法鉴定艰难梭菌的效果。方法随机选取2013年1月至2015年1月南安市医院收治住院患者,抽取其中50例的腹泻粪便标本,均分离出艰难梭菌,同时根据检测方法的不同将其分为研究组(25例)、对照组(25例)。对照组采用24 h培养法进行鉴定检测,研究组采用PCR扩增tcd B基因法鉴定,比较两组临床艰难梭菌鉴定结果。结果研究组中,临床经PCR扩增tcd B基因法鉴定后,明显提升临床鉴定艰难梭菌的阳性率,与对照组比较存在统计学意义(P<0.05)。结论应用PCR扩增tcd B基因法鉴定,效果优于24 h培养法鉴定,可将PCR扩增tcd B基因法作为临床艰难梭菌鉴定的重要方法。
作者 洪亚华
出处 《临床医学研究与实践》 2016年第2期71-71,共1页 Clinical Research and Practice
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