北五味子总木脂素对环磷酰胺诱导的免疫功能低下小鼠的调节作用

Immunomodulatory effect of Schisandra chinensis lignans in cyclophosphamide-induced immunodeficiency mice

目的:研究北五味子总木脂素(SCL)对环磷酰胺(Cyp)所致免疫抑制小鼠的免疫调节作用,并阐明其作用机制。方法:75只雄性ICR小鼠随机分为对照组,模型组,低、中和高剂量(50、100和200mg·kg-1)SCL组,每组15只。对照组和模型组小鼠灌胃给予同体积的蒸馏水,各剂量SCL组小鼠灌胃给予相应剂量的SCL,连续21d。在给药第17天,模型组和各剂量SCL组小鼠腹腔注射Cyp (20mg·kg-1),对照组小鼠腹腔注射同体积的生理盐水,连续5d。给药结束后,测定各组小鼠胸腺和脾脏指数,并观察胸腺和脾脏组织形态表现,采用碳粒廓清测试法评估巨噬细胞的吞噬功能,采用ELISA法测定γ干扰素(IFN-γ)水平,采用MTT法评价脾淋巴细胞增殖能力,采用流式细胞术检测脾淋巴细胞凋亡率。结果:与对照组比较,模型组小鼠脾脏和胸腺指数、巨噬细胞吞噬指数、IFN-γ水平及淋巴细胞增殖能力明显降低(P<0.05或P<0.01),淋巴细胞早期、晚期和总凋亡率明显升高(P<0.01)。与模型组比较,低、中和高剂量SCL组小鼠脾脏和胸腺指数,巨噬细胞吞噬指数,IFN-γ水平,淋巴细胞增殖能力,淋巴细胞早期、晚期及总凋亡率均明显升高(P<0.05或P<0.01)。与对照组比较,模型组小鼠胸腺皮质淋巴细胞数量减少,脾小体数量和体积明显减少,呈萎缩状态。与模型组比较,各剂量SCL组小鼠胸腺皮质淋巴数量减少、脾小体减少和萎缩均有一定程度改善。结论:SCL可通过调节小鼠细胞、体液和非特异性免疫功能对Cyp诱导的免疫功能低下小鼠起到保护作用。

Objective:To investigate the immunomodulatory effect of Schisandra chinensis lignans(SCL)in the cyclophosphamide(Cyp)-induced immunosuppressive mice, and to elucidate its related mechanisms.Methods:A total of 75 male ICR mice were divided in control group,model group,and low,middle,high doses(50,100,and 200 mg·kg-1)of SCL groups,15 mice in each group.The mice in control and model groups were administered with distilled water intragastrically and the mice in SCL groups were administered with different doses of SCL intragastrically once a day for 21 d.After the successive administration for 17 d,the mice in model and SCL groups were intraperitoneally injected with Cyp(20 mg·kg-1)once a day for 5 d.The thymus and spleen indexes were determined and the morphology of thymus and spleen tissues were observed.The phagocytic function of macrophages was estimated using carbon clearance test and the level of interferon-γ(IFN-γ)was measured by ELISA method.The spleen lymphocyte proliferation ability was measured by MTT method and the apoptotic rate of spleen lymphocyte was determined by flow cytometry.Results:Compared with control group,the thymus and spleen indexes,the phagocytic function of macrophages,the IFN-γlevel,the spleen lymphocyte proliferation activity,and the early,late,total apoptotic rates of lymphocytes of the mice in model group were increased(P<0.05 or P<0.01).Compared with model group,the thymus and spleen indexes,the phagocytic function of macrophages,the IFN-γlevels,the spleen lymphocyte proliferation abilities,and the early,late,total apoptotic rates of lymphocytes of the mice in different doses of SCL groups were significantly decreased(P<0.05 or P<0.01).Compared with control group,the number of lymphocytes in the thymus cortex of the mice in model group was reduced,the number of splenic corpuscles was reduced and their volumes were obviously reduced.Compared with model group,the reducing of lymphocyte number in the thymus cortex and splenic corpuscles and atrophy of the mice in different doses of SCL groups were obviously improved.Conclusion:SCL can protect the mice with Cypinduced immunodeficiency by regulating the cellular,humoral and non-specific immune functions of the mice.