沉默信息调节因子1在结直肠癌组织中的表达及意义

Expression and significance of Sirtuin 1 in human colorectal cancer

目的 研究沉默信息调节因子1（SIRT1）在结直肠癌组织及癌旁正常组织中的表达情况及其与临床病理特征的关系.方法 分别用实时荧光定量PCR（RT-QPCR）技术及免疫组织化学（IHC）技术检测45例结直肠癌组织及30例癌旁正常组织中SIRT1在mRNA及蛋白水平上的表达情况,分析其与临床病理特征的关系.结果 SIRT1 mRNA在结直肠癌组织中的表达量较正常组织高,其中位数（四分位数间距）分别为2.488（3.447）、1.563（2.867）,差异有统计学意义（Z=-2.304,P＜0.05）.SIRT1蛋白水平在结直肠癌组织及正常组织中的阳性表达率分别为71.1％（32/45）和43.3％ （13/30）,差异有统计学意义（x2 =5.787,P=0.029）.且SIRT1的表达水平与结直肠癌的淋巴结转移（RT-QPCR：Z=-2.160,P=0.031;IHC：P =0.043）、肿瘤分期（RT-QPCR：Z=-2.411,P=0.016;IHC：P=0.008）相关,与组织分化程度可能相关（RT-QPCR：x2=10.864,P=0.004;IHC：P =0.322）,而与患者的年龄、性别、肿瘤大小、大体类型、浸润深度、肿瘤部位及远处转移无关.结论 SIRT1在结直肠癌组织中呈现高表达,其可能作为一种致癌基因参与结直肠癌的发生、发展,并可能影响患者的预后.

Objective To research the expressions of Sirtuin 1 （SIRT1） in colorectal cancer and normal tissue and the relationship with clinicopathologic features.Methods The expression levels of SIRT1 mRNA and protein were detected respectively by quantitative real-time reverse transcriptase PCR （RT-QPCR） and immunohistochemistry （IHC） in 45 cases of colorectal carcinoma and 30 cases of normal mucous membrane tissue,and the relationships between the expression status and clinicopathologic features were analyzed.Results In colorectal carcinoma,the expression of SIRT1 mRNA were higher than that in control group [M（QR）：2.488（3.447） vs.1.563（2.867）,Z =-2.304,P ＜0.05],so was the protein level （71.1％ vs.43.3％,x2 =5.787,P =0.029）.The expression of SIRTI was associated with lymph node metastasis （RT-QPCR：Z =-2.160,P =0.031;IHC：P =0.043）,neoplasm stages （RT-QPCR：Z =-2.411,P =0.016;IHC：P =0.008）,and might associated with the tissue differentiation （RT-QPCR：x2 =10.864,P =0.004;IHC：P =0.322）.On the other hand,no significant differences were observed regarding age,sex,tumor size,gross type,depth of invasion,tumor site and distant metastasis.Conclusion The expression of SIRT1 in colorectal cancer is higher than that in control group,the high level of SIRT1 is related to the tumorigenesis and progression of colorectal cancer as well as correlated with prognosis in colorectal cancer.