鸡血藤血清联合顺铂对人肺癌PG细胞增殖及周期影响的研究

Effect of Caulis Spatholobi Containing Serum and Cisplatin on the Proliferation Cycle of Human Lung Cancer Cell Line PG

[目的]探讨鸡血藤血清对人肺癌PG细胞株的增殖抑制及周期阻滞作用。[方法]将肺癌PG细胞分为空白组、鸡血藤组、鸡血藤+顺铂组、顺铂组,四唑盐比色法(MTT)实验中各组根据血清浓度的不同分为20%、10%、5%、2.5%、4组,观察各组PG细胞的增殖抑制作用;HE染色法、免疫荧光法和流式细胞术实验中各组血清浓度为20%,采用光镜和激光共聚焦显微镜观察各组PG细胞形态,流式细胞仪检测细胞周期。[结果]MTT法中5%、10%、20%鸡血藤组细胞的增殖抑制明显,具有时间和剂量依赖性,但低于顺铂组和鸡血藤+顺铂组,鸡血藤+顺铂组PG细胞的增殖抑制率与顺铂组比较,差异无统计学意义(P>0.05)。光镜和激光共聚焦显微镜下鸡血藤组细胞的凋亡程度明显,但低于顺铂组和鸡血藤+顺铂组。与空白组比较,鸡血藤组PG细胞的G_2/M期比例增加(P<0.05);顺铂组和鸡血藤+顺铂组的S和G_2/M期比例增加(P<0.01),G_0/G_1期降低(P<0.01)。顺铂组和鸡血藤+顺铂组较鸡血藤组的S期和G_2/M期比例增加(P<0.01),G_0/G_1期降低(P<0.01)。鸡血藤+顺铂组PG细胞的各期与顺铂组比较,差异无统计学意义(P>0.05)。[结论]鸡血藤血清通过引起细胞周期G_2/M期阻滞,从而抑制细胞增殖,这与顺铂阻滞细胞于G_2/M和S期不同。

[Purpose] To investigate the effect of Caulis Spatholobi（JXT） containing serum on the proliferation cycle of human lung cancer cell line PG. [Methods] The lung cancer PG cells were divided into four groups ：the blank control group, the JXT group, the JXT plus cisplatin group, and cisplatin group. The inhibiting effect of cell proliferation in 20%,10%,5%,2.5% serum concentration groups was observed by MTT method. The morphology of PG cells in 20% serum concentration group was observed using light microscope and laser scan-ning confocal microscope.The cell cycle arrest was observed using flow cytometry in 20% serum concentration group.[Results] Under the MTT method, the inhibiting effect of PG cells in the 5%,10%,20% JXT group was significant and had a time-dose dependence, but was less than that of the JXT plus cisplatin group as well as the cisplatin group.Compared with the cisplatin, proliferation inhibiting rate of PG cells in the JXT＋DDP group had no significant differences（P〈0.05）. Under the light microscope and laser scanning confocal microscope,the apoptosis degree of PG cells in the JXT group was significant,but was less than that of the JXT plus cisplatin group as well as the cisplatin group. Compared with the blank control group,the proportion of PG cells increased at G2/M phases（P〉0.05） in the JXT group;the proportion of PG cells increased at G2/M and S phases（P〈0.05,P〈0. 01） and decreased at G0/G1 phase（P〈0.01） in the JXT plus cisplatin group as well as the cisplatin group.Compared with the JXT group,the proportion of PG cells increased at G2/M and S phases（P〈0.01）and decreased at G0/G1phase（P〈0.01） in the JXT plus cisplatin group as well as the cisplatin group.There was no statistical difference in PG cells at each phase between the cisplatin group and the JXT plus cisplatin group（P〉0.05）.[Conclusion] JXT containing serum inhibit PG cell proliferation by G2/M phase arresting, which is different from the manner of arresting PG cells at G2/M and S phases in cisplatin.