抗感冒药中对乙酰氨基酚含量的红外光谱法测定

Determination of Acetaminophen in Anti-cold Drugs by Internal Standard Method of FT-IR

建立了红外光谱内标法测定抗感冒药中对乙酰氨基酚的方法。选择铁氰化钾作为内标物,定量添加到对乙酰氨基酚中,在对乙酰氨基酚百分含量为0.16%~1.67%范围内,测得混合物红外光谱中对乙酰氨基酚测量峰(1564.1cm^(-1))和铁氰化钾内标峰(2117.6cm^(-1))的峰高比(y=h_i/h_s)与两者质量比(x=m_i/m_s)有良好的线性关系,其线性回归方程为y=0.8448x+0.0842,相关系数r=0.9983。据此测得抗感冒药样品中对乙酰氨基酚的含量,测定结果与药典法相近。

A novel method for the determination of acetaminophen in anti-cold drugs by internal standard method of Fourier transform infrared spectrophotometry（FT-IR） was establlished. Potassium ferricyanide was added into acetaminophen as internal reference and the mixtures were detected by FT-IR. The peak of acetaminophen at（1564±1）cm^-1 was chosen as quantitative peak and the one of potassium ferricyanide at（2117±1） cm^-1 was chosen as inside mark quantitative peak. The ratio of two peak＇s absorbance（y=hi/hs） had good linear relationship with their mass ratio（x=mi/ms） when the mass fraction of acetaminophen was 0.16%-1.67%. The linear regression equation was y=0.8448x＋0.0842, and the linear correlation coefficient was 0.9983. The contents of acetaminophen in the anti-cold drugs sample was detected and the determination results were similar to those of pharmacopoeia method.