茶树根际球囊霉素相关土壤蛋白含量及其与土壤因子的关系

Contents of Glomalin-related Soil Protein and Its Correlations with Soil Factors in the Rhizosphere of Tea Plant [Camellia Sinensis(L.) O. Kuntze]

球囊霉素相关土壤蛋白（Glomalin-related soil protein,GRSP）是由丛枝菌根真菌（Arbuscular mycorrhizal fungi,AMF）产生的一种含金属离子的糖蛋白,对维护AMF本身的生理功能以及保持土壤有机碳平衡和土壤团聚体稳定性极为重要.于贵州4个重点茶区（湄潭县、石阡县、贵定县和都匀市）茶园采集茶树根际土样,采用Bradford法测定茶树根际GRSP,并分析土样的基本理化性质,以了解茶树根际GRSP含量及其与土壤因子的关系.结果表明,茶树根际土壤总GRSP（Total glomalin-related soil protein,T-GRSP）和易提取GRSP（Easily extractable glomalin-related soil protein,EE-GRSP）含量因茶树品种、种植区域不同而各异,总体含量分别在5.71-22.84 mg·g^-1和2.35-7.91 mg·g^-1间,均值分别为12.96 mg·g^-1和4.88 mg·g^-1.相关性分析发现,T-GRSP与水解氮（Hydrolysable nitrogen,Nh）、速效钾（Available potassium,Ka）和有机质（Organic matter,OM）含量极显著正相关;EE-GRSP与Nh、Ka和OM含量极显著正相关,与土壤pH值显著负相关.多元线性回归方程表明,不同土壤因子对GRSP的贡献不同,对于T-GRSP的贡献,水解氮〉速效磷〉有机质;对于EE-GRSP的贡献,水解氮〉速效磷.可见,茶树根际GRSP含量丰富,它与土壤因子有密切关系,可通过了解GRSP的含量来评价茶树根际土壤质量.

Glomalin-related soil protein (GRSP) is a kind of glycoprotein containing metal ions that are secreted by arbuscular mycorrhizal fungi (AMF). It plays an important role in maintaining physiological functions of AMF and stabilization of soil organic carbon and soil aggregate. In this paper, the contents of GRSP and its correlations with soil factors were analyzed in the rhizosphere of tea plant [Camellia Sinensis(L.) O. Kuntze]. Total glomalin-related soil protein (T-GRSP) and easily extractable glomalin-related soil protein (EE-GRSP), as well as soil factors were investigated by Bradford and conventional soil factors analysis method in four key tea planting areas (Meitan county, Shiqian county, Guiding county and Douyun city) of Guizhou province, southwest China. The results showed that GRSP levels were dependent on both rhizospheres of different tea cultivars and planting areas. The range of T-GRSP and EE-GRSP were 5.71-22.84 mg·g-1 and 2.35-7.91 mg·g-1, with average of 12.96 mg·g-1 and 4.88 mg·g-1 respectively. Correlation analysis showed that T-GRSP was significant positive correlation with hydrolysable nitrogen (Nh), organic matter (OM) and available potassium (Pa). EE-GRSP was positive correlated with Nh, Pa and OM, but negative correlated with soil pH. The result of multiple linear regression equation showed that different soil factors showed different impact on the content of T-GRSP and EE-GRSP. For T-GRSP: Nh > Pa > OM. For EE-GRSP: OM > Pa. These results showed the GRSP was remarkably abundant in the rhizosphere of tea plant and closely related with soil factors, so the content of GRSP may be an appropriate index for evaluating soil quality in tea garden.