甲苯胺红不加热血清试验阳性与自身免疫性疾病的相关性研究

Correlation of TRUST syphilis positive result with autoimmune diseases

目的：探究甲苯胺红不加热血清试验（tolulized red unheated serum test,TRUST）阳性与自身免疫性疾病的关系,为临床研究提供参考依据。方法：选择2010年5月至2014年6月期间来我院诊治的100例红斑狼疮、类风湿性关节炎等自身免疫性疾病患者为病例组,分别采用TRUST和酶联免疫吸附法（ELISA）检测,比较两种方法的阳性检测率。同期选择100例健康人群为对照组,比较病例组和对照组人群的抗心磷脂抗体（ACA）浓度的高低。结果：采用ELISA检测病例组和对照组ACA浓度,病例组患者的Ig G-ACA、Ig M-ACA与β2-GP1的水平均显著高于对照组人群,具有明显差异性（P〈0.05）;经过TRUST检测,实验组患者凝集反应强度为3＋~4＋者35例,2＋者45例,1＋者15例,-者为5例,阳性率95.0%,显著高于对照组患者,具有明显差异性（P〈0.05）。结论：患有自身免疫性疾病的患者的Ig G-ACA、Ig MACA与β2-GP1等ACA水平显著高于正常人群,是一项值得重视的检测指标。TRUST对于筛选自身免疫性疾病阳性患者,具有一定的特异性,具有较高的检查率,可以在临床上使用。

Objectives： To explore the toluidine red unheated serum test（ TRUST） positive relationship with autoimmune diseases,to provide reference for clinical research. Methods： 100 cases of lupus,rheumatoid arthritis and other autoimmune disease from May 2010 to June 2014 in our hospital were selected as the case group.The positive detection rates of TRUST and ELISA were compared. 100 healthy people in the same period were selected as control group. Anti- cardiolipin antibodies（ ACA） concentration levels of case group and control population were compared. Results： Through the use of enzyme- linked immunosorbent assay（ ELISA） to test ACA concentration of case and control groups,Ig G- ACA,Ig M- ACA and β2- GP1 levels of case group were significantly higher than these of control group,with significant differences（ P〈0. 05）; 1 after TRUST testing,agglutination intensity of case group were 35 cases of 3 ＋- 4 ＋,45 cases of 2 ＋,15 cases of 1 ＋,5 cases of-,the positive rate was 95. 0%,which was significantly higher than that in control group（ P〈0. 05）. Conclusion： The levels of Ig G- ACA,Ig M- ACA and β2- GP1 and other anti- cardiolipin antibodies（ ACA） in patients with autoimmune disease were significantly higher than these in normal population,which are noteworthy detection indexes. Toluidine red unheated serum test（ TRUST） has some specificity in screening positive for patients with autoimmune disease,and have a higher positive rate,which is worth wide application.