粒细胞集落刺激因子对百草枯干预小鼠黑质纹状体通路的影响

Influence of Granulocyte-colony Stimulating Factor on Nigrostriatal System of Paraquat Treated Mice

目的探讨粒细胞集落刺激因子(G-CSF)对百草枯干预小鼠脑内黑质纹状体通路的影响。方法 C57BL/6小鼠随机分为百草枯组、G-CSF干预组和对照组(均n=8)。高效液相色谱分析测定纹状体多巴胺含量,同时采用免疫组化ABC法观察黑质部酪氨酸羟化酶(TH)阳性表达神经元。结果腹腔注射百草枯20 mg·kg^(-1)·d^(-1),连续5 d后,小鼠自发性活动明显减少,纹状体多巴胺含量明显减少,黑质部TH阳性表达明显减少,与对照组比较差异有统计学意义(P<0.05);给予G-CSF干预后纹状体多巴胺含量和黑质部TH阳性表达明显增加,与百草枯组比较差异有统计学意义(P<0.05)。结论 G-CSF干预后小鼠纹状体的多巴胺含量升高,黑质部TH阳性表达增加,提示G-CSF对小鼠黑质纹状体通路多巴胺能神经元可能具有一定的保护作用。

Aim To explore the influence of granulocyte-colony stimulating factor（G-CSF） on nigrostriatal system of parkinsonian mice induced by paraquat. Methods C57BL/6 mice were divided into three groups randomly which were paraquat treated group, G-CSF treated group and the control group. High performance liquid chromatography（HPLC） was used to measure the content of dopamine in striatum. At the same time, immunoreactivity of tyrosine hydroxylase（TH） positive neurons in substantia nigra of mice was estimated using immunohistochemistry. Results After i.p. injection of mice with paraquat（20 mg·kg-（-1）） daily for 5 days, the content of dopamine in striatum and immunoreactivity of TH positive neurons in substantia nigra of mice decreased compared to that of the mice treated by saline injected intraperitoneally. In the mice treated with daily subcutaneously injections of G-CSF 100 μg·kg-（-1） for 5 days, the content of dopamine in striatum and immunoreactivity of TH positive neurons in substantia nigra of mice increased compared to that of paraquat group mice. Conclusion Injecting G-CSF subcutaneously could cause the content of dopamine in striatum and the immunoreactivity of TH positive neurons in substantia nigra increase in parkinsonian mice induced by paraquat, which indicated that G-CSF may have neuroprotective effect on dopaminergic neurons of nigrostriatal system.