桑色素分子印迹传感器的制备与应用

Fabrication and Application of a Molecularly Imprinted Sensor for Morin Detection

以邻氨基酚（o-AP）为功能单体,桑色素为模板分子,基于分子间的相互作用力,在金电极表面电聚合制备具有特异性识别孔穴的桑色素分子印迹传感器膜。采用循环伏安法（CV）、差分脉冲伏安法（DPV）等研究了分子印迹膜的性能和分子印迹效应。探索了聚合膜配比及聚合扫描圈数对传感器性能的影响,优化了洗脱时间和印迹时间。比较了此传感器对其结构相似物的选择性响应,发现其对桑色素检测具有良好的选择性。在最佳实验条件下,此传感器对桑色素浓度定量测定范围为0.05~1.70μmol/L,线性方程为I（μA）=1.0800lgc（mol/L）＋9.3599,R=0.9934,检出限为0.01μmol/L。用此传感器测定黑茶样品中桑色素的含量,加标回收率为104.0%~108.0%。

Based on the intermolecular interaction,a molecularly imprinted polymer electrochemical sensor with specific identification of cavity on gold electrode surface was proposed with o-aminophenol as functional monomers and morin as template molecule. The performance and effect of MIP were investigated by cyclic voltammetry（ CV） and differential pulse voltammetry（ DPV）. Factors affecting the properties of sensor,such as polymeric membrane ratio,scanning cycles,elution time and adsorption time were investigated. Substances which had a similar structure to morin was used to compared the selective response. The result showed that the sensor had a good selectivity to morin. Under the optimized experiment conditions,the current response of the imprinted sensor was linear to the concentration of morin in the range of 0. 05- 1. 7 μmol / L with linear equation as follows： I（ μA） = 1. 0800lgc（ mol / L） ＋ 9. 3599（ R = 0. 9934）,and the detection limit of0. 1 μmol / L. The sensor was used to determine the content of morin in black tea samples,and the recoveries of standard addition were between 104. 0% and 108. 0%.