FeCl_3溶液诱导葡萄愈伤组织白藜芦醇积累及其与氧化应激的关系

The Relationship between FeCl_3-induced Resveratrol Accumulation and Oxidative Stress in Grapevine Callus

【目的】研究葡萄白藜芦醇的诱导剂,为开发新的葡萄保鲜剂与农药提供参考。【方法】采用Fe Cl3水溶液对红地球葡萄叶片愈伤组织进行诱导,应用HPLC分析愈伤组织中白藜芦醇的含量变化,DAB染色法检测活性氧水平,使用RT-PCR方法分析茋合酶基因(STS)的表达量变化。【结果】Fe Cl3溶液对葡萄愈伤组织茋类物质具有显著的诱导作用,诱导白藜芦醇的积累呈现显著的量时依赖性;1.6 m M Fe Cl3溶液处理18 h后愈伤组织中白藜芦醇含量可提高约8.5倍,鲜重达到210.8μg/g。Fe Cl3溶液处理组的活性氧水平均高于对照组;加抗氧化剂CAT和NAC可明显降低Fe Cl3对白藜芦醇诱导效果。【结论】Fe Cl3溶液对红地球葡萄叶愈伤组织白藜芦醇积累具有显著升高作用,其积累机制与Fe Cl3所导致的氧胁迫正相关。

【Objective】To find a resveratrol elicitor on grape,present study takes an preliminary work for developing antistaling agents in grape storage.【Method】The detached grape leaf callus（ Vitis vinifera L. cv.Red Globe） were treated with different concentrations of Fe Cl3 aqua solution. The content of resveratrol in samples was analyzed by HPLC assay. The biosynthetic regulation of stilbenes was determined as the quantity of stilbene synthase（ STS） by realtime PCR methods. ROS levels were detected with DAB stain method.【Result】The results showed that resveratrol accumulated significantly in both dose- and time- dependent manners with Fe Cl3 treatments. Furthermore,the maximum content of resveratrol reached 210. 8 μg / g achieved by1. 6 m M plus 18 hours treatment in treated grape callus,whose content is was 8. 5- fold higher than untreated samples. It showed that ROS levels in the Fe Cl3 treatments were higher than those untreated. ROS scanvagers CAT and NAC significantly reduced resveratrol content in Fe Cl3 treatments.【Conclusion】Therefore,Fe Cl3 solution has significantly increased resveratrol accumulation in the grape leaf callus via an accumulation mechanism positive related to oxidative stress.