应用大型溞评价铜内胆中饮用水的急性毒性

The detection of acute toxicity effect of the drinking water reserved in coppery tank with Daphnia Magna

目的评价市售某饮水机铜内胆中饮用水对大型溞的急性毒性效应,计算其半数有效浓度(EC50)和半数致死浓度(LC50)。方法以大型溞为模式生物,参照GB/T 16125—2012《大型溞急性毒性实验方法》,考察不同浓度的铜内胆饮用水对大型溞运动受抑制和死亡的影响,分析该饮水机铜内胆的化学元素组成以及试验水样中金属离子的浓度,探讨金属离子浓度与急性毒性效应之间的联系。结果该试验饮水机铜内胆中饮用水对大型溞24 h时EC50和LC50分别为5.55%和8.32%,48 h时EC50和LC50分别为3.58%和5.61%,铜内胆饮用水中铜和铅的浓度分别是GB 5749—2006《生活饮用水卫生标准》相应限值的10.51倍和1.17倍,铜内胆中饮用水的急性毒性效应随着大型溞暴露浓度的升高而增强。结论铜内胆中饮用水对大型溞有一定的急性毒性效应,使用时应注意健康防护。

Objective To evaluate the acute toxicity of the drinking water reserved in a commercially available coppery tank with Daphnia Magna,and to calculate its half effective concentration（ EC50） and half lethal concentration（ LC50）. Methods Taking Daphnia Magna as model organism,the inhibition and deaths effect of Daphnia Magna were investigated by reference to Method for Acute Toxicity Test of Daphnia Magna Straus（ GB / T 16125—2012）. The coppery tank elementary analysis and metal ions concentration measurement were conducted simultaneously during the experimentation to explore the interaction between metal ions concentration and acute toxicity effect. Results It was revealed that the 24 h and 48 h EC50 for Daphnia Magna were5. 55% and 3. 58%,while the LC50 for Daphnia Magna were 8. 32% and 5. 61%,respectively. The concentrations of Cu and Pb in the drinking water were 10. 51 and 1. 17 times over the national standard separately,and the acute toxicity effect was gradually rising up with the increasing exposure concentration of the Daphnia Magna. Conclusion In some way,the drinking water reserved in coppery tank has a certain toxicity for Daphnia Magna,and more attentions must be focused on when using it.