2型糖尿病患者嘌呤能受体水平检测及其与C-反应蛋白的相关性研究

Research on the correlation of P2X7 receptor levels with C-reactive protein in patients with type 2 diabetes

目的探讨2型糖尿病患者外周血单核细胞中嘌呤能受体(P2X7R)的表达变化及其与血清C-反应蛋白(CRP)和细胞因子(TNF-α、IL-10、IL-1β)变化的相关性。方法选取38例2型糖尿病患者作为实验组(其中20例CRP正常患者,18例CRP升高患者),20例健康体检者为正常对照组,通过免疫比浊法检测CRP,酶联免疫吸附法(ELISA)检测血清细胞因子(TNF-α、IL-10、IL-1β)水平;并采用实时荧光定量PCR(RT-PCR)法检测外周血单核细胞P2X7R的mRNA表达水平(以β-actin为内参)。结果 2型糖尿病组患者的CRP、TNF-α及IL-1β水平显著高于正常对照组,P2X7R基因的表达水平也显著高于正常对照组,差异均有统计学意义(P<0.05)。CRP升高2型糖尿病患者的TNF-α、IL-1β的水平明显高于CRP正常2型糖尿病组患者,而CRP升高2型糖尿病组患者的IL-10水平显著低于其他2组,差异均有统计学意义(P<0.05)。结论单核细胞表达的P2X7R基因是2型糖尿病患者的重要危险因子,与CRP水平无关,在2型糖尿病的发生及进展过程中可能具有重要作用。

Objective To investigate the expression changes of P2X7 R from peripheral blood mononuclear cells in patients with type 2 diabetes and the relationship with the chances of serum C-reactive protein（ CRP） and serum cytokines（ TNF-α,IL-10 and IL-1β）. Methods 38 cases of type 2 diabetes patients（ including 20 patients with normal CRP and 18 patients with high CRP） and 20 healthy subjects were collected as the experimental group and the control group. The CRP was assayed by immunoturbidimetry,cytokines（ TNF-α,IL-10 and IL-1β） were assayed by enzyme linked immunosorbent assay（ ELISA）.Meanwhile the P2X7 expression in peripheral blood monocytes was detected by real-time PCR（ RT-PCR）. Results The levels of CRP and serum cytokines（ TNF-α,IL-1β） in type 2 diabetes patients were significantly higher than those in the control group,while the expression level of P2X7 in patients with type 2 diabetes group was significantly higher than that in the control group,and there was statistical significance on the difference between the groups（ P 0. 05）. The levels of serum cytokines（ TNF-α,IL-1β） in type 2 diabetes patients were significantly higher than those in the control group,in addition,in increased CRP type 2 diabetes group,IL-10 level significantly decreased compared with the other 2 groups,and the difference was statistically significant（ P 0. 05）. Conclusion The levels of serum cytokines and P2X7 expression in monocyte are major risk factors in patients with type 2 diabetes,which was not related with the CRP. There are likely to be a relevant mechanism in the occurrence and progress of T2 DM patients.