晚期血吸虫病患者外周血中Vα24自然杀伤T细胞水平观察

Observation on Vα24 natural killer T cells level in peripheral blood in patients with advanced schistosomiasis

目的探讨晚期血吸虫病患者外周血中Vα24自然杀伤T细胞(NKT)水平及其与肝纤维化的关系。方法采用流式细胞术,检测41例晚期血吸虫病患者和48例健康体检者(对照组)外周血中的Vα24 NKT细胞占T淋巴细胞的比例;同时对晚期血吸虫病患者进行腹部超声检查,根据超声检查结果进行肝纤维化分级,比较各级间NKT细胞水平。结果 41例晚期血吸虫病患者的NKT细胞平均水平为(0.25±0.10)%,较健康对照组[(1.32±0.56)%]明显下降,差异有统计学意义(P<0.01)。晚期血吸虫病患者肝纤维化越严重,NKT细胞减少越明显,1级、2级、3级患者NKT细胞水平分别为(0.38±0.03)%、(0.29±0.04)%、(0.16±0.03)%,各级患者比较差异均有统计学意义(P<0.01)。结论晚期血吸虫病患者外周血中Vα24 NKT细胞水平明显下降,并随肝纤维化程度加重而减少,晚期血吸虫病患者发生及肝纤维化进展可能与Vα24 NKT细胞水平低下和/或功能缺陷有关。

Objective To investigate the change in the level of Vα24 natural killer T（ NKT） cells in peripheral blood and its correlation with the severity of hepatic fibrosis in patients with advanced schistosomiasis. Methods Flow cytometry was used to detect the proportion of Vα24 NKT cells in peripheral blood of 41 patients with advanced schistosomiasis and 48 healthy subjects（ control group）. At the same time,the patients with advanced schistosomiasis were examined by abdominal ultrasonography,and the liver fibrosis was graded according to the results of ultrasound examination. The levels of NKT cells were compared between all levels. Results The average percentage of Vα24 NKT cells in 41 cases of advanced schistosomiasis patients was（ 0. 25 ± 0. 10） %,which was significantly lower than that in healthy control groups（ 1. 32 ± 0. 56） %,and the difference was significant（ P 0. 01）. The more severe liver fibrosis in patients with advanced schistosomiasis,the more obvious the NKT cells decreased. The percentages of NKT cells in 1,2 and 3 grade were respectively（ 0. 38 ± 0. 03） %,（ 0. 29 ± 0. 04） % and（ 0. 16± 0. 03） %. There were significant differences in all levels of patients（ P 0. 01）. Conclusion The level of Vα24 NKT cells in peripheral blood of patients with advanced schistosomiasis decreased significantly,and decreased with the degree of hepatic fibrosis. Progression of advanced schistosomiasis patients and the progression of liver fibrosis may be related to the low level of Vα24 NKT cells and / or functional defects.