黄芩苷纳米结构脂质载体制备及大鼠心脏缺血/再灌注保护研究

Preparation of Baicalin Nanostructured Lipid Carriers and Their Protective Effects Against Heart Ischemia/Reperfusion Injury in Rats

目的:制备黄芩苷纳米结构脂质载体,并考察对大鼠心脏缺血/再灌注损伤的保护作用。方法:采用热熔乳化超声-低温固化法制备黄芩苷纳米结构脂质载体,并考察其包封率、粒径分布、Zeta电位、微观形态及体外释放行为;考察黄芩苷纳米结构脂质载体对大鼠心脏缺血/再灌注损伤的保护作用。结果:黄芩苷纳米结构脂质载体的包封率为(92.1±3.6)%,平均粒径为(241.6±52.2)nm,Zeta电位为(-33.7±2.2)m V,透射电镜显示黄芩苷纳米结构脂质载体成圆整、规则球形。黄芩苷纳米结构脂质载体中药物的体外释放符合Higuchi方程(Q=0.5316t^(1/2)+3.5415,r=0.9590)。黄芩苷纳米结构脂质载体可以增加大鼠心脏缺血/再灌注损伤的保护作用。结论:黄芩苷纳米结构脂质载体对大鼠心脏缺血/再灌注损伤具有良好的保护作用。

Objective：To prepare Baicalin nanostructured lipid carriers,and study their protective effects against heart ischemia/reperfusion injury in rat. Methods：The Baicalin nanostructured lipid carriers were prepared by melt-emulsion ultrasonication and low temperature-solidification methods. The encapsulation efficiency（EE%）,particle size distribution,Zeta potential,morphology and in vitro release were examined,respectively. The Baicalin nanostructured lipid carriers protected against heart ischemia/reperfusion injury in rats were studied. Results：For encapsulation efficiency（EE%）,particle size distribution,zeta potential of nanostructured lipid carriers were found to be（92.1±3.6）%,（241.6±52.2）nm and（-33.7±2.2）m V,respectively. The Baicalin nanostructured lipid carriers were found to be small and spherical with smooth surface as seen in transmission electron microscopy. Drug release profile in vitro was in accordance with Higuchi equation（Q=0.5316t^（1/2）＋3.5415,r=0.9590）. Baicalin nanostructured lipid carriers could increase protected effectively against heart ischemia/reperfusion injury in rats. Conclusions：Baicalin nanostructured lipid carriers protected effectively against heart ischemia/reperfusion injury in rats.