肝脂肪酶基因-514C/T多态性与陕西地区非酒精性脂肪肝人群关系研究

Association of hepatic lipase gene promoter polymorphism-514C/T with nonalcoholic fatty liver disease of Shanxi

目的:比较陕西地区人群中非酒精性脂肪肝(NAFLD)患者与正常人群肝脂肪酶(HLG)-514C/T多态性分布差异,探讨其与NAFLD发病易感性关系。方法:选取NAFLD患者96例及正常对照组98例,用非变性聚丙烯酰胺凝胶电泳进行基因多态位点检测HLG-514位点基因型,并测量其体重指数、腰臀比、血压、胆固醇、高密度脂蛋白、低密度脂蛋白、甘油三酯、空腹血糖指数。结果:正常对照组HLG-514CC、CT、TT基因型分别是26.5%、59.2%、14.2%,NAFLD组31.3%、63.5%、5.2%,两组CC基因型频率和C等位基因频率比较有统计学差异。结论:肝脂肪酶-514C/T以杂合子C/T基因型所占的百分比高,其基因多态性与陕西NAFLD患者发病有关。

Objective：To study the prevalence of the hepatic lipase gene（LIPC）promoter polymorphism（at position-514）in patients of Shanxi with nonalcoholic fatty live disease（NAFLD）,and its relationship with the susceptibility to NAFLD.Methods：Genotype of LIPC promoter was detected with non-denaturant polyacrylamide gel electrophoresis in 96 patients with NAFLD and 98 of normal control.Body mass index,waist-to-hip ratio（WHR）,blood pressure,CHOL,HDL,LDL,TG and FPG of the patients were measured.Results：The proportions of genotypes of CC、CT and TT at-514 of LIPC in healthy people were26.5%、59.2%、14.2%,respectively,whereas those in patients with NAFLD were 31.3%、63.5%、5.2%,The frequency of CC genotype and C allele in the NAFLD group were significantly higher than those in the control group.Conclusion：The proportions of genotypes of C/T are dominant at-514 of LIPC in patients with NAFLD and controls.Gene polymorphism of LIPC-5 14C/T is correlated with susceptibility to NAFLD of Shanxi.