不同施灸方向对寒凝血瘀证大鼠血液流变学的影响（英文）

Effects of different directions of moxibustion therapy on hemorheology in rats with blood stasis due to cold retention

目的:观察不同施灸方向对寒凝血瘀证模型大鼠血液流变学的影响。方法:将32只Wistar大鼠按随机数字表随机分成4组,分别为正常组、模型组、顺经灸组和逆经灸组,每组8只。除正常组以外,其余3组制备大鼠寒凝血瘀证模型。造模成功后,对顺经灸组和逆经灸组两组大鼠背部的膀胱经经段实施不同方向的艾灸治疗,每天艾灸10 min,7天为1疗程,治疗2个疗程,共计14 d。观察大鼠症状和体征量化评分变化,每星期评分1次,共计7次。治疗结束后,检测大鼠血液流变学指标。结果:造模结束后,模型组、顺经灸组和逆经灸组大鼠的症状和体征量化评分明显高于正常组(P<0.05)。治疗结束后,模型组大鼠症状和体征量化评分明显高于正常组(P<0.05);顺经灸组和逆经灸组大鼠症状和体征量化评分明显低于模型组(P<0.05),顺经灸组大鼠症状和体征量化评分明显低于逆经灸组(P<0.05)。模型组大鼠全血粘度、红细胞聚集指数较正常组大鼠明显升高(P<0.05),但红细胞压积和血沉升高不明显(P>0.05);顺经灸组和逆经灸组大鼠全血粘度、红细胞聚集指数均显著低于模型组大鼠(P<0.05),顺经灸组和逆经灸组两组差异无统计学意义(P>0.05)。结论:顺经灸及逆经灸对寒凝血瘀证大鼠血液流变学以及大鼠寒凝血瘀的症状均有不同程度的改善,顺经灸对大鼠寒凝血瘀症状改善优于逆经灸,顺经灸与逆经灸对大鼠血流变学影响无明显差异。

Objective： To investigate the effects of different directions of moxibustion therapy on hemorheology in rat models with blood stasis due to cold retention. Methods： A total of 32 Wistar rats were randomly divided into 4 groups, based on the random digits table, including a normal group, a model group, a moxibustion along the meridian group, and a moxibustion against the meridian group, with 8 rats in each group. Except the normal group, the other 3 groups were used to make the rat models with blood stasis due to cold retention. Rats in the moxibustion along and against the meridian groups accepted moxibustion therapy in different directions on the trunk segment of the Bladder Meridian after successful modeling. Moxibustion for 10 min every day, and 7 d as a course of treatment. Two courses of treatment（a total of 14 d） were carried out. Quantitative score of signs and symptoms change was observed once a week, with a total of 7 times. Hemorheological indexes of rats were detected when the treatment finished. Results： The quantitative scores of signs and symptoms in the model, moxibustion along the meridian and against the meridian groups were significantly higher than those in the normal group after modeling（P〈0.05）. When the treatment finished, the quantitative score of signs and symptoms in the model group was significantly higher than that in the normal group（P〈0.05）; the quantitative scores of signs and symptoms of rats in the moxibustion along and against the meridian groups were significantly lower than that in the model group（P〈0.05）; the quantitative score of signs and symptoms in the moxibustion along the meridian group was significantly lower than that in the moxibustion against the meridian group（P〈0.05）; the whole blood viscosity and erythrocyte aggregation index in the model group were significantly higher than those in the normal group（P〈0.05）, however the increases of erythrocyte hematocrit and erythrocyte sedimentation rate were not obvious（P〈0.05）; whole blood viscosity and erythrocyte aggregation index in the moxibustion along and against the meridian groups were significantly lower than those in the model group（P〈0.05）, while there were no statistically significant differences between the moxibustion along the meridian group and moxibustion against the meridian group（P〈0.05）. Conclusion： Moxibustion along and against the meridian both showed different degrees of improvement for hemorrheology and symptoms of blood stasis due to cold retention. Improvement for symptoms of blood stasis due to cold retention was better in the moxibustion along the meridian group than that in the moxibustion against the meridian group. The influence on rat hemorrheology showed no significant difference between moxibustion along the meridian and moxibustion against the meridian.