受四环素影响的活性污泥胞内外聚合物特征

The characteristics of extracellular polymeric substances and intracellular polymeric substances in activated sludge fed with tetracycline

利用离心加超声的方法对处理四环素抗生素废水的活性污泥胞外聚合物(EPS)和胞内聚合物(IPS)进行提取,将其分为-溶解型胞外聚合物(slime)、松散附着型胞外聚合物(LB)、紧密附着型胞外聚合物(TB)、胞内聚合物(IPS).用BCA法和蒽酮-硫酸法测定了各层EPS和IPS溶液中的蛋白质和多糖含量,同时用三维荧光光谱扫描(EEM)和同步荧光光谱扫描(SFS)对其中的溶解性有机物(DOM)进行定性和定量分析,结果表明在EPS中的蛋白质和多糖的含量高于IPS,且蛋白质含量均比多糖含量高,而在slime和IPS中的蛋白质和多糖的含量高于LB和TB;低激发波长类色氨酸和高激发波长类色氨酸是EPS和IPS溶液的主要成分,且溶液中的溶解性代谢产物和芳香蛋白类物质相对含量在slime、LB、TB和IPS逐渐增加,而难降解有机物-腐殖酸类物质的相对含量逐渐降低;进水四环素浓度与污泥各层EPS和IPS溶液中DOM也具有较好的相关性;三维荧光法检测溶液中的DOM与化学分析测试出的蛋白质含量具有较好的相关性.

Extracellular polymeric substances（EPS） and intracellular polymeric substances（IPS） of activated sludge feeding with tetracycline were divided into four parts, soluble extracellular polymeric substances（slime）, loosely bound EPS（LB）, tightly bound EPS（TB）, intracellular polymeric substances（IPS） after extractionby centrifuge and ultrasonic process. Protein and polysaccharide were detected with BCA and anthrone-sulfuric acid method. At the same time, 3Dexcitation-emission spectral scan（EEM） and synchronous fluorescence spectrum scan（SFS） were applied to quantitatively and qualitatively analyze DOM in EPS and IPS. The results showed that the content of protein and polysaccharide in EPS was higher than that in IPS, and the content of protein was higher than that of polysaccharide. In slime and IPS, the content of protein and polysaccharide was higher than that in LB and TB. Tryptophan-like with low and high excitation wavelength was the main component of EPS and IPS. The content of microbial byproducts and aromatic proteins increased gradually in slime, LB, TB and IPS, while the refractory organic humic-like decreased. Influent tetracycline concentration has good correlation with DOM of EPS and IPS from activated sludge. And the DOM detected by EEM had good correlation with protein detected by chemical test.