摘要
目的:观察不同浓度苗药痛风停汤兔含药血清对人肾小管上皮细胞尿酸盐转运体(h UAT)mRNA和尿酸盐阴离子转运体1(h URAT1)mRNA表达的影响。方法:建立肾小管上皮细胞模型,分为苗药痛风停汤高、中、低剂量组和苯溴马隆组、空白组;采用清洁级新西兰制备含药血清家兔,以高、中、低剂量苗药痛风停汤兔含药血清、苯溴马隆兔含药血清、0.9%氯化钠注射液兔含药血清干预,采用实时荧光定量PCR检测HK-2细胞中h UAT、h URAT1 mRNA的相对表达量(双标准曲线法)。结果:与空白组比较,苗药痛风停汤高、中、低剂量组h UATmRNA表达水平均增高,h URAT1mRNA表达水平均下降,差异均有统计学意义(P<0.05)。结论:苗药痛风停汤对体外培养的HK-2细胞h UAT、h URAT1mRNA的表达有调控作用,在mRNA水平上上调h UAT、下调h UART1的表达可能是苗药痛风停降尿酸的作用机制之一。
Objective: To observe the effects of rabbit serum containing different concentrations of Miao medicine Tongfengting decoction on the mRNA expression of a human urate transporter / channel( h UAT) and the human urate transporter 1( h URAT1) in human renal tubular epithelial cells. Methods: The renal tubular epithelial cells were divided into high-,medium-,and low- dose Miao medicine Tongfengting decoction groups,benzbromarone group,and blank group. The drug- containing serum was collected from CL New Zealand rabbits. The cells in the above groups were treated with rabbit serum containing high-,medium-,and low- dose Miao medicine Tongfengting decoction,benzbromarone,and 0. 9% sodium chloride solution,respectively. Quantitative real- time PCR was used to determine the relative mRNA expression of h UAT and h URAT1 in HK- 2 cells( based on double standard curves). Results:Compared with the blank group,the high-,medium-,and low- dose Miao medicine Tongfengting decoction groups had significantly elevated mRNA expression of h UAT and significantly reduced mRNA expression of h URAT1( P〈0. 05). Conclusion: Miao medicine Tongfengting decoction regulates the mRNA expression of h UAT and h URAT1 in HK- 2 cells in vitro. The upregulation of h UAT and downregulation of h URAT1 at the mRNA level are likely to play a role in reduction of uric acid by Miao medicine Tongfengting decoction.
出处
《湖南中医杂志》
2016年第2期167-169,共3页
Hunan Journal of Traditional Chinese Medicine
基金
贵州省科学技术联合基金(编号:黔科合中药字[2012]LKZ7013)
贵州省中医药管理局中医药
民族医药科学技术项目(编号:QZYY-2014-007)