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Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses 被引量：2

Comparison of lentiviruses pseudotyped with S proteins from coronaviruses and cell tropisms of porcine coronaviruses
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摘要 The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins(S) were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus(PEDV) and transmissible gastroenteritis virus(TGEV) have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected VeroCCL-81(monkey kidney), Huh-7(human liver), and PK-15(pig kidney) cells efficiently. CCL94(cat kidney) cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening. The surface glycoproteins of coronaviruses play an important role in receptor binding and cell entry. Different coronaviruses interact with their specific receptors to enter host cells. Lentiviruses pseudotyped with their spike proteins（S） were compared to analyze the entry efficiency of various coronaviruses. Our results indicated that S proteins from different coronaviruses displayed varied abilities to mediate pseudotyped virus infection. Furthermore, the cell tropisms of porcine epidemic diarrhea virus（PEDV） and transmissible gastroenteritis virus（TGEV） have been characterized by live and pseudotyped viruses. Both live and pseudoviruses could infected VeroCCL-81（monkey kidney）, Huh-7（human liver）, and PK-15（pig kidney） cells efficiently. CCL94（cat kidney） cells could be infected efficiently by TGEV but not PEDV. Overall, our study provides new insights into the mechanisms of viral entry and forms a basis for antiviral drug screening.
作者 Jingjing Wang Feng Deng Gang Ye Wanyu Dong Anjun Zheng Qigai He Guiqing Peng
机构地区 State Key Laboratory of Agricultural Microbiology College of Veterinary Medicine The Cooperative Innovation Center for Sustainable Pig Production
出处 《Virologica Sinica》 SCIE CAS CSCD 2016年第1期49-56,共8页 中国病毒学（英文版）
基金 supported by the National Natural Science Foundation of China (Grant No.31372440)
关键词 病毒学 临床 医学 药物学 Coronavirus spike proteins receptor binding cell entry pseudotyped virus
分类号 S852.65 [农业科学—基础兽医学]
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Virologica Sinica
2016年 第1期

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