关于在远洋船员中开展丙型肝炎病毒核酸(HCV-RNA)检测的研究探讨

Discussion of HCV- RNA detection among the ocean crew

目的通过调查天津口岸远洋船员的抗HCV-IgG阳性率和HCV RNA检测阳性率的符合度,了解处于传染期的远洋船员占抗HCV-IgG阳性船员的概率。并证明利用酶联免疫吸附实验对远洋船员这一处于狭小、封闭空间的特殊人群进行HCV筛查的局限性,探讨使用更灵敏、准确的技术对船员进行HCV检查,以防止船员由于彼此的密切接触而增加感染风险的重要性。方法利用RT-PCR方法检测38份抗HCV-IgG阳性血清标本和76份阴性血清标本,此标本来自于2012年度于天津国际旅行卫生保健中心进行《国际旅行健康检查证明书》办理的远洋船员。结果 RT-PCR检测结果表明,受检船员中,抗HCV-IgG阳性者RNA检测阳性率为34.21%,抗HCV-IgG阴性者RNA检测阳性率为1.31%。结论由于ELISA方法本身的局限性、HCV感染病程和远洋船员生活环境的特殊性,导致使用酶联免疫吸附实验检测具有一定的局限性,因此需要辅以RNA检测。

Objective This article investigates the compliance between the positive ratio of anti- HCV IgG and the positive ratio of HCV RNA detection among the ocean crews at Tianjin Port. We demonstrate the limitation of enzyme- linked immunosorbent assay（ ELISA） for HCV screening among ocean crews,a particular population living in small and confined spaces. We also discuss the importance of utilizing more sensitive and accurate techniques to perform HCV screening for ocean crews,in order to prevent the increase of risk of HCV infection by close contact with each other. Methods RT- PCR method are used to detect 38 anti- HCV IgG positive and 76 negative serum samples which is collected from ocean crews by the Tianjin International Travel Health Care Center in 2012.Results RT- PCR test results show that the positive ration of the anti- HCV IgG RNA is 34. 21% and the negative ration is 1. 31%. Conclusion Due to the limitations of ELISA method itself,HCV- infection course and the particularity of the ocean crews＇ living environment,the ELISA method test result is relatively limited,and RNA detection should be adopted as the supportive method.