浅析3种梅毒血清检测方法在临床诊断中的应用

The application of 3 methods for detecting syphilis in clinical diagnosis

目的研究分析3种梅毒血清检测方法在临床诊断中的应用效果。方法选取本中心2014年5月-2015年4月期间采集并明确诊断为梅毒患者98例血样标本,并另行抽取来我中心体检所采集的120例健康人员血样标本进行对照检验,全部血样均分成3等份。结果 ELISA方法的阳性检出率为96.9%、TPPA阳性检出率为99.0%,TRUST的阳性检出率仅为83.7%明显低于另外两种方式（χ^2=9.9462,P=0.0016）差异有统计学意义。结论 ELISA与TPPA方法在梅毒血清检测中具有较高的检出率,可作为梅毒血清学检测的首选手段。

Objective This article studies the application effect of 3 kinds of syphilis serum detection method in clinical diagnosis.Methods A total of 98 cases syphilis patients＇ blood samples collected during April,2014,to May,2015 are selected,and another 120 cases of healthy blood samples collected by the physical examination center are chosen.All the samples are divided into three equal groups.Results The positive rate is 96.9%by ELISA method;the positive rate is 99.0%by TPPA method;and the positive detection rate is only 83.7%by TRUST method,which is significantly lower than that of the other two methods（χ^2 =9.9462,P=0.0016）.Conclusion The ELISA and TPPA methods have a high detection rate in the detection of syphilis serum,which can be used as the first choice for the serological detection of syphilis.