摘要
目的观察高迁移率族蛋白B1(HMGB1)及Toll样受体2(TLR2)在哮喘小鼠肺内的表达,及1,25-二羟基维生素D3(1,25-(OH)_2D_3)对二者表达的影响。方法将30只BALB/c小鼠随机分为3组:对照组、哮喘组和干预组,应用HE染色方法观察各组小鼠肺组织形态学改变,并测定相同级别支气管气道壁厚度,免疫组化法观察HMGB1、TLR2的表达,RT-PCR方法测定各组小鼠肺内HMGB1mRNA及TLR2mRNA的表达。结果哮喘组小鼠肺内HMGB1及TLR2的表达均高于对照组,干预组二者的表达明显低于哮喘组,差异均有统计学意义(P<0.05);哮喘组气道壁厚度高于对照组及干预组,差异有统计学意义(P<0.05)。结论 HMGB1和TLR2参与了哮喘的发病过程;1,25-(OH)_2D_3能抑制HMGB1及TLR2在哮喘小鼠肺内的表达,且在具有抑制哮喘小鼠气道重塑的作用。
Objective To observe the expressions of high mobility group box 1（ HMGB1） and Toll-like receptor 2（ TLR2） in lung of mice with asthma and the effect of 1,25-（ OH）2D3on expressions of the two factors. Methods Thirty BALB / c mice were randomly divided into control group,asthma group,and intervention group. HE staining was used to observe morphological changes of lung tissue,the thicknesses of airway wall of the same degree were measured,immunohistochemical method was used to observe the expressions of HMGB1 and TLR2,RT-PCR was used to detect the expressions of HMGB1 mRNA and TLR2 mRNA in lung. Results The expression levels of HMGB1 and TLR2 in lung of asthma group were higher than those in control group,the expression levels of HMGB1 and TLR2 in lung of intervention group were lower than those in control group,there were statistically significant differences（ P〈0.05）; the thicknesses of airway wall of the same degree in asthma group were statistically significantly higher than those in control group and intervention group（ P〈0.05）.Conclusion HMGB1 and TLR2 participate in the pathogenesis of asthma; 1,25-（ OH）2D3can inhibit the expressions of HMGB1 and TLR2 in lung and airway remodeling of asthmatic mice.
出处
《中国妇幼保健》
CAS
2016年第6期1273-1276,共4页
Maternal and Child Health Care of China
