长链非编码RNA CRNDE对胶质瘤细胞增殖和迁移的影响

Lnc RNA Colorectal Neoplasia Differentially Expressed(CRNDE) Regulated Cell Proliferation and Migration in Glioma

目的探讨CRNDE在胶质瘤细胞中发挥的功能和可能的作用机制。方法将CRNDE基因干扰si RNA转染人胶质瘤U87细胞系,利用CCK8和Transwell实验分别检测细胞增殖和迁移能力变化,并利用Real-time PCR研究下游基因表达情况。结果 si RNA干扰CRNDE表达的胶质瘤细胞中,细胞增殖和迁移能力明显降低,MAPK/ERK通路中关键蛋白激酶Raf1、MEK2、ERK1、ERK2基因及转录因子c-Myc、NF-κB基因的表达均有显著降低。结论 CRNDE可能通过调节MAPK/ERK通路一系列相关基因表达,增加胶质瘤细胞的增殖和迁移能力。

Objective To investigate the function and mechanism of colorectal neoplasia differentially expressed（CRNDE） in glioma cell line. Methods After transfected with CRNDE si RNAs, cell proliferation of glioma cell line U87 was assessed by CCK8 assay and cell migration was performed using Transwell assay. We also used Real-time PCR assays to detect the downstream genes expression. Results The abilities of cell proliferation and migration were decreased in si RNA-transfected glioma cell lines. The expression of the key kinases（Raf1, MEK2, ERK1, ERK2） and transcription factors（c-Myc, NF-κB） in MAPK/ERK pathway were down-regulated. Conclusion CRNDE could enhance the abilities of cell proliferation and migration in glioma through activating related gene expression in MAPK/ERK pathway.