Ti-6Al-7Nb喷砂酸蚀处理后对大鼠成骨细胞的影响

The effects of sanding acid etch treatment of Ti-6Al-7Nb surface on rat osteoblasts

目的研究纯钛(Ti)、Ti-6Al-7Nb表面经喷砂酸蚀(SLA)处理后大鼠成骨细胞的生物相容性。方法实验分为Ti机械打磨组(S1组)、Ti喷砂酸蚀组(SLA1组)、Ti-6Al-7Nb机械打磨组(S2组)以及Ti-6Al-7Nb喷砂酸蚀组(SLA2组)。用显微镜观察4组样品的表面形貌,使用接触角测量仪分析SLA1、SLA2组样品表面亲水性,并将2组样品放入模拟体液(SBF)中,7、14、21 d后分别用扫描电镜(SEM)、X线衍射仪(XRD)观察样品表面沉积物的形貌、物相,将从大鼠颅盖骨中提取的成骨细胞接种于4组样品上,用倒置显微镜观察大鼠成骨细胞形态,用MTT比色法测量大鼠成骨细胞增殖情况。结果显微镜下SLA1、SLA2组较S1、S2组样品表面拥有更多孔洞,接触角测量仪分析得出SLA1、SLA2组样品表面为亲水性结构,且SLA2组接触角小于SLA1组;SEM显示在14 d时SLA2组样品表面最先观察到覆盖的羟基磷灰石涂层,21 d时2组样品表面都观察到羟基磷灰石涂层。MTT实验结果经统计学分析得出成骨细胞在SLA1、SLA2组样品表面增殖能力强于S1、S2组,且在SLA2组样品表面的增殖能力强于SLA1组(P<0.05)。结论喷砂酸蚀处理的Ti-6Al-7Nb拥有良好的生物相容性,有利于促进种植体与骨组织的结合。

Objective To investigate the biocompatibility of pure titanium（Ti） and Ti- 6Al-7Nb surface treated bysanding acid etch（SLA） on rat osteoblasts. Methods Experiments were divided into four groups, Ti mechanical grindinggroup（S1 group）, Ti sand-blasting acid group（SLA1 group）, Ti-6Al-7Nb mechanical grinding group（S2 group） and Ti-6Al-7Nb sand-blasting acid group（SLA2 group）. The surface topography of samples was examined by microscope. The contactangle measurement instrument was used to analyse surface hydrophily of SLA1 and SLA2 groups. The surface sediment mor-phology and phase were observed by scanning electron microscopy（SEM） and X-ray diffraction（XRD） in two groups after be-ing soaked into simulated body fluid（SBF） for 7 d,14 d and 21 d. Osteoblasts extracted from rats were seeded on titaniumsheets, and the osteoblast cells on different titanium surfaces were observed by inverted microscope. MTT colorimetric meth-od was used to measure the proliferation of osteoblasts. Results Compared with S1 and S2 groups, there were more holes onsample surface of SLA1 and SLA2 groups. The sample surface was hydrophilic structure in SLA1 and SLA2 groups. The con-tact angle was smaller in SLA2 group than that of SLA1 group. The hydroxyapatite coating was firstly observed in SLA2 group at 14 d. The hydroxyapatite coating was found in samples of two groups after 21 d. The proliferative ability of osteo-blasts was stronger in SLA1 and SLA2 groups than that of S1 and S2 groups. And the proliferative ability of osteoblasts wasstronger in sample surface of SLA2 group than that of SLA1 group（P〈0.05）. Conclusion Ti-6Al-7Nb by SLA has goodbiological compatibility, which is helpful to promote the combination of implant and bone tissue.