江苏省阜宁地区自然人群中18～45岁女性人乳头瘤病毒16、18型中和抗体及DNA的流行率

Detection of neutralizing antibodies and DNA of human papillomavirus 16, 18 in women aged 18-45 years in Funing, Jiangsu province

目的 研究江苏省阜宁地区自然人群中18～45岁女性人乳头瘤病毒（HPV）16、18型中和抗体及DNA的分布。方法 2012年12月至2013年1月,采用多阶段随机抽样的方法,在江苏省阜宁地区共招募1 494名18～45岁女性,采集宫颈脱落细胞进行HPV DNA检测,采集血清并通过假病毒中和试验检测抗HPV16、18型中和抗体,采用SPSS 21.0软件分析不同年龄段HPV DNA阳性率及HPV中和抗体阳性率的差异。结果 1 494名女性中HPV16型 DNA阳性28例（1.9%）、中和抗体阳性188例（12.6%）;HPV18型 DNA阳性15例（1.0%）、中和抗体阳性60例（4.0%）。HPV16、18型 DNA和中和抗体阳性率在各年龄段的分布差异均无统计学意义（P〉0.05）。16.7%的研究对象已感染过HPV16和/或18型。结论 江苏省阜宁地区自然人群中18～45岁女性大多数未感染过HPV16、18型,是HPV16、18型疫苗应用的目标人群。

Objective To understand the distributions of DNA and neutralizing antibodies of human papillomavirus （HPV）16, 18 in 18-45 year-old women. Methods Totally, 1 494 women were enrolled through multistage random sampling in Funing, Jiangsu province. Cervical exfoliated cells were collected from them for HPV DNA testing, and serum samples were taken from them for the detection of HPV16, 18 neutralizing antibodies by using pseudovirion-based neutralization assay （PBNA）. Results Among the 1 494 women, 28（1.9%） and 188（12.6%） were positive for DNA and neutralizing antibody of HPV16 respectively, and 15（1.0%） and 60（4.0%） were positive for DNA and neutralizing antibody of HPV18, respectively. There were no significant differences in the detection rates of DNA and neutralizing antibody of HPV16, 18 among different age groups. About 16.7% of the women were infected with HPV16, 18, or both. Conclusion In Funing county of Jiangsu province, most women aged 18-45 years has no immunity to HPV16 and 18, indicating that they are appropriate targets for HPV 16/18 vaccination.