三结构域蛋白29在人胃癌组织的表达及其临床意义

Expression of tripartite motif - containing protein 29 in gastric cancer tissue and its clinical significance

目的探讨三结构域蛋白29（TRIM29）在胃癌组织中的表达及其临床意义。方法收集86例胃癌及癌旁组织，检测组织中TRIM29mRNA及蛋白的表达量，分析TRIM29表达水平与胃癌患者临床病理参数及预后的关系。结果胃癌组织与癌旁组织中TRIM29mRNA的相对表达量分别为1．490±0．059和1．010±0．079，两者比较差异有统计学意义（P〈0．01），且随着胃癌临床分期增高和肿瘤浸润程度加重而上调；TRIM29与有无淋巴结转移和患者预后情况差异有统计学意义（P〈0．05），而与性别、年龄和肿瘤分化程度无相关（P〉0．05）。结论胃癌组织中TRIM29表达显著上调，其高表达与肿瘤的肿瘤浸润程度、TNM分期和有无淋巴结转移有关。

Objective To investigate the expression of tripartite motif - containing protein 29 （TRIM29） in gastric cancer tissues, and its relationship with clinicopathological parameters. Methods We examined RNA and protein levels of TRIM29 expression in 86 paired stomach tumor and paratumor specimens by real -time fluorescent quantitative polymerase chain reaction （FQ -PCR） and Western blotting. The relationship of the TRIM29 expression with clinicopathological parameters and prognosis of gastric cancer patients was analyzed. Results The relative expression of TRIM29 was 1. 490± 0. 059 and 1. 010 ±0. 079 in gastric cancer tissues and normal adjacent tissues respectively, with significant difference （P 〈 0. 05）. Higher mRNA and protein expression of TRIM29 in gastric cancer was associated with depth of invasion and TNM stage, while not with gender, age and differentiation degree （P 〉 0.05 ）. Patients with lymphatic metastasis got higher Trim29 expression than in those with no lymphatic metastasis. Conclusion Expression levels of TRIM29 mRNA and protein in gastric cancer tissue are significantly up - regulated. TRIM29 expression is associated with invasion depth and TNM stages.