蛇床子素对机械性脑损伤小鼠的治疗作用研究

Therapeutic effects of osthole in mice following stab wound injury

目的:研究蛇床子素对机械性脑损伤模型小鼠的治疗作用。方法:利用针刺法在小鼠大脑人字缝前2 mm、中线左侧2 mm建立小鼠机械性脑损伤模型。小鼠随机分为假手术组、模型组和蛇床子素10,20和30 mg·kg-1治疗组。用HE染色法观察各组在7 d之后的大脑皮层结构;酶联免疫检测试剂盒(ELISA)法检测损伤部位的IL-6和TNF-α炎症因子表达情况;免疫组织化学染色法检测大脑皮层及海马区神经元的数量;利用免疫荧光染色法检测凋亡的神经元数量。结果:10,20和30 mg·kg-1蛇床子素治疗组均能够改善损伤的大脑皮层结构;对IL-6和TNF-α炎症因子的表达有明显的抑制作用;显著增加大脑皮层及海马区神经元的数量;明显减少神经元的凋亡。结论:蛇床子素对机械性脑损伤的小鼠具有一定的治疗作用,可能是通过抑制炎症因子的表达和减少神经元的凋亡等。

Objective： To investigate the therapeutic effects of osthole on brain stab wound injury in mice.Methods： The mice underwent stab wound injury by a needle,then were randomly divided into sham operation group,model group,and osthole 10,20,30 mg·kg^- 1treatment groups. The structures of cerebral cortexes were observed with HE staining after 7 days. ELISA technique was used to assay the expressions of IL-6 and TNF-α in the injury site. Immunohistochemistry staining was used to detect the number of neurons in cerebral cortex and hippocampus. The apoptosis of neurons was assayed by immouostaining. Results： Treatment with osthole 10,20,30mg·kg- 1significantly improved the structure of cerebral cortex,and obviously inhibited the expressions of IL-6 and TNF-α. Osthole could significantly increase the number of neurons in cerebral cortex and hippocampus and decrease the apoptosis of neurons. Conclusion： Osthole has therapeutic effects on stab wound injury in mice,and the possible mechanism may be inhibiting the expressions of inflammatory factors and reducing the apoptosis of neurons.