对瓜类疫霉菌诱导响应的黄瓜WRKY转录因子筛选研究

Screening of CsWRKYs Involved in Phytophthora Blight Resistance in Cucumber(Cucumis sativus)

以黄瓜感病品种‘B80’为材料并通过RT-qPCR技术,针对黄瓜全基因组54个CsWRKYs基因,在瓜类疫霉菌侵染后对其进行表达分析,并在水杨酸(SA)和茉莉酸甲酯(Me JA)处理下对受瓜类疫霉菌诱导(或抑制)基因进行表达检测,分析其可能参与的抗病相关信号通路。研究结果表明:15个CsWRKYs基因(CsWRKY3、11、13、21、22、23、26、31、34、41、44、46、48、52和56)受瓜类疫霉菌诱导上调表达,仅CsWRKY6受瓜类疫霉菌抑制下调表达。在SA和MeJA处理下,7个Cs WRKYs基因(Cs WRKY26、34、41、46、48、52和56)受SA诱导,CsWRKY44同时受SA和MeJA诱导,CsWRKY6受Me JA抑制下调表达,推测7个基因(CsWRKY26、34、41、46、48、52和56)可能通过SA介导的信号通路调控植物对病原菌的响应;CsWRKY6可能通过JA介导的信号通路调控植物防御反应过程;CsWRKY44则可能同时参与SA和JA介导的抗病相关信号通路。

By RT-qPCR technology, the expression patterns of 54 CsWRKYs in Phytophthora melonis（P. melonis）-susceptible cucumber cultivar ‘B80＇ were analyzed after inoculation with P. melonis and then P. melonis-induced（suppressed） CsWRKYs were analyzed under the treatment of salicylic acid（SA） and methyl jasmonate（Me JA） to investigate their signaling pathways. The results in this study showed that fifteen CsWRKYs（ CsWRKY3, 11, 13,21, 22, 23, 26, 31, 34, 41, 44, 46, 48, 52 and 56） were strongly induced and only CsWRKY6 suppressed by P.melonis. Among them, seven CsWRKYs（ CsWRKY26, 34, 41, 46, 48, 52 and 56） were SA-inducible and up-regulated while CsWRKY6 was down-regulated after treatment of Me JA. CsWRKY44 was commonly up-regulated by both SA and Me JA. Based on the results above, we predicted CsWRKY26, 34, 41, 46, 48, 52 and 56 might be involved in Phytophthora blight resistance in cucumber by SA and（or） JA signaling pathway（s）.