整树结香法所产沉香的GC-MS分析

GC-MS Analysis of the Agarwood Produced by Whole-tree Agarwood-Inducing Technology

采用整树结香法诱导健康白木香树结香,并根据树体受伤后木材的颜色形态特征将木材分为腐烂层、沉香层和白木层,运用气相色谱-质谱联用(GC-MS)技术分别对三层的乙醚提取物进行了分析。结果表明,不同层化学成分的种类和相对含量上有明显差异。沉香层与腐烂层的2-(2-苯乙基)色酮类成分总相对含量都很高,腐烂层的色酮类成分相对含量(70.25%)高于沉香层(60.08%),但沉香层的色酮种类(14个)比腐烂层(10个)多;倍半萜类成分在沉香层和腐烂层中的相对含量均较低,沉香层的倍半萜类成分相对含量(5.75%)略高于腐烂层(3.75%),而腐烂层的倍半萜个数(11个)略多于沉香层(8个)。白木层则以甾体类化合物为主,没有检出色酮类和倍半萜类成分。

In th is paper, the agarwood was induced to form in the healthy Aquilaria sinensis with whole-tree agarwood-inducing technology. Then the wood was divided into three sections, which respectively named rotten layer, agarwood layer, and whitewood layer, according to their color and morphological characteristics. The chemical constituents of the three layers were analyzed by using gas chromatography- mass（ GC-MS） spectrometry. The results showed that different types and relative content of the chemical components were found in different layers.Compared with the relative content of 2-（2-phenylethyl） chromone derivatives in the agarwood layer（60.08%）, it was more abundant in the rotten layer（ 70.25%）, however, the former had more 2-（2-phenylethyl） chromone derivatives（14） than the latter（10）. Meanwhile, the relative content of sesquiterpenes was rather low in both the agarwood layer and the rotten layer. The agarwood layer had slightly more content（5.75%） than the rotten layer（3.75%）, while the rotten layer（11） had a little more sesquiterpenes than the agarwood layer（8）. Unlike other two layers, the whitewood layer mainly contains steriods instead of 2-（2-phenylethyl） chromone derivatives or sesquiterpenes.