长茎羊耳蒜菌根真菌类群的研究

Mycorrhizal community of Liparis viridiflora

研究了广西雅长自然保护区和云南西双版纳自然保护区共3个产地的兰科植物羊耳蒜属长茎羊耳蒜Liparis viri-diflora的菌根真菌类群区系组成。根内菌根真菌的核糖体基因内转录间隔区序列（r DNA-ITS）采用PCR技术扩增、克隆、测序并构建系统发育树。结果表明,长茎羊耳蒜根内所检测到的真菌大部分为胶膜菌科Tulasnellaceae真菌;根据序列相似性和系统发育分析,所有真菌可归为12个可操作分类单元（OTU）,其中胶膜菌科有7个OTUs,达到总数的90.6%,为优势类群。菌根真菌多样性及区系组成在3个不同产地样本之间存在一定的差异;菌根真菌可能和兰科植物的生境适应性存在一定的相关性。

The mycorrhizal fungal community composition of Liparis viridiflora collected from 3 habitats in Yachang of Guangxi and Xishuangbanna of Yunnan as we investigated. Ribosomal DNA internal transcribed spacer（r DNA-ITS） sequences of mycorr-hizal fungi were amplified, cloned, sequenced and phylogenetically analyzed. The results suggested that most of the detected fungi belong to Tulasnellaceae. Based on the sequence similarity and phylogenetic analysis, all the fungi were divided into 12 operational taxonomic units（OTUs）, of which 7 OTUs were species of Tulasnellaceae, accounting for 90.6% of the total se-quences. Mycorrhizal fungal diversity and communities in the 3 different habitats showed certain differences. This result indi-cated that mycorrhizal fungi might correlate with habitat adaption of orchid plants.