摘要
[目的]建立测定浓香型白酒中乳酸含量的HPLC方法。[方法]采用Agilent SB-C18柱(4.6×150 mm^2,5μm)色谱柱,0.005 mol/L H2SO4溶液为流动相,流速1.0 m L/min,检测波长210 nm,柱温35℃,进样量20μL。[结果]乳酸在1~12 mg/m L的浓度范围内与峰面积呈良好线性关系(R2=0.999 8),平均回收率为99.36%(RSD=0.066%),最低检出限为0.009 mg/m L。[结论]此方法准确、简便、快速,适用于浓香型白酒中乳酸的含量测定。
[Objective]To establish a HPLC method for the determination of the lactic acid content in Chinese strong aromatic spirits.[Methods]An Agilent SB-C 18 column (4.6×150 mm2, 5μm) was used with sulfate acid as the mobile phase at a concentration of 0.005 mol/L. The flow rate was 1.0 mL/min and the detection wave length was 210 nm.The temperature of the column was 35 ~C and the sample amount was 20μL.[Results]There was a good linear relationship between the concentration of lactic acid within 1-12 mg/mL and the peak area (R2=0.999 8 ).The average recovery rate was 99.36% (RSD=0.066%).The lowest detection limit was 0.009 mg/mL.[Conclusion]This method could be acCurate,simple,quick and suitable for the determination of lactic acid in Chinese strong aromatic spirits.
出处
《现代农业科技》
2016年第4期291-292,共2页
Modern Agricultural Science and Technology
基金
河南省重点科技攻关(152102110156)
南阳理工学院青年基金(NGKY-2014-12)
关键词
浓香型白酒
乳酸
含量测定
高效液相色谱法
Chinese strong aromatic spirits
lactic acid
content determination
HPLC