紫草提取物有效成分测定及对骨骼肌自由基代谢和抗疲劳能力影响的实验研究

Experimental study on the determination of gromwell extract and its influence on the metabolism of free radical in skeletal muscle and anti-fatigue effect

目的:通过建立小鼠游泳和大鼠跑台2个训练实验模型,研究紫草提取物对骨骼肌自由基代谢的影响以及抗疲劳作用;并测定紫草提取物中的有效成分。方法:选取雄性小鼠和大鼠各24只,将小鼠随机分为游泳训练组(a组)、游泳力竭组(b组)和游泳加药组(c组),每组各8只;将大鼠随机分为跑台训练组(A组)、跑台力竭组(B组)、跑台加药组(C组),每组各8只。小鼠和大鼠按照不同的训练模型分别进行游泳和跑台训练,在训练方案的最后1天,a、A组动物不进行训练,在安静状态下处死制备待测样本,并通过试剂盒测定骨骼肌超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、过氧化氢酶(CAT)、巯基(-SH)、丙二醛(MDA)指标;b、B、c、C组动物进行一次力竭运动,力竭后即刻处死并进行样本处理和上述指标测定。结果:训练对照组(b、B组)动物在游泳和跑台力竭运动后,骨骼肌组织抗氧化酶活性显著下降(P<0.05),自由基代谢产物MDA含量显著升高(P<0.05);补充紫草提取物组(c、C组)动物在力竭运动后,与训练对照组动物(b、B)相比,骨骼肌组织抗氧化酶活性显著升高(P<0.05),自由基代谢产物MDA含量显著降低(P<0.05),运动至力竭的时间明显延长(P<0.05)。结论:外源性补充紫草提取物可以改善骨骼肌组织自由基清除和代谢水平,延缓运动疲劳的产生。

Objective：To explore the influence of gromwell extract on the metabolism of free radicals in skeletal muscle and its anti-fatigue effect by establishing two training models of mouse swimming and rat treadmill.Methods：Totally 24 male mice and 24 male rats were selected.Mice were divided into 3 groups—swimming training group（group a）,exhausted swimming group（group b）,swimming and medicine group（group c）,and rats were divided into 3 groups—treadmill training group（group A）,exhausted treadmill group（group B）,treadmill training and medicine group（group C）,with 8 mice or rats in each group.Mice and rats were trained by different training schemes.At the last day of training,group a and group A were not trained as usuall and were sacrificed at resting state to prepare test samples,and the determination of indexes SOD,GSH-Px,CAT,-SH and MDA in skeletal muscle was conducted using the reagent box.Groups b,c,B and D were trained by exhausting exercise and then were sacrificed immediately after being exhausted to prepare test samples and determine the above indexes.Results：After the exhausting exercise of swimming and treadmill,the anti-oxidant enzyme activity of skeletal muscle tissue was decreased greatly（p〈0.05） and the content of MDA produced by free radical metabolism was significantly increased（p〈0.05） in groups b and B.Compared with groups b and B,the anti-oxidant enzyme activity of skeletal muscle tissue of groups c and C was increased greatly（p〈0.05）,the content of MDA produced by free radical metabolism was significantly decreased（p〈0.05）,and the time of exercise to exhaustion was significantly prolonged.Conclusion：Exogenous gromwell extract supplement can improve the balance of free radical metabolism and prolong the exhaustive time of endurance-trained mice and rats.