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HPLC指纹图谱技术在傣药灯台叶资源评价中的应用 被引量:4

Application of HPLC fingerprint technology in the resource evaluation of Alstonia scholaris(L.) R.Br.
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摘要 目的:建立不同产地灯台叶HPLC指纹图谱,为灯台叶产地鉴别、质量控制和评价提供科学依据,推动傣药现代化发展进程。方法:采用正交试验设计优化灯台叶超声提取方法,建立HPLC指纹图谱。色谱条件:采用Agilent ZORBAX Eclipse XDB C18色谱柱(250 mm×4.6 mm,5μm),以0.1%甲酸水(A)-乙腈(B)为流动相,梯度洗脱(0~5 min,5%B;5~35 min,5%B→42%B;35~40 min,42%B→80%B;40~50 min,80%B→95%B;50~65 min,95%B),流速1.0 m L·min^(-1),柱温30℃,检测波长287 nm,进样量5μL。结合相似度评价及聚类分析对不同产地灯台叶指纹图谱进行比较。结果:正交实验结果表明,70%甲醇水溶液超声50 min,料液比1∶10为最佳提取条件。建立了灯台叶HPLC指纹图谱,确定了10个共有峰。海南中和、广西南宁北湖村、海南海口高新区等3个产地样品相似度小于0.8,系统聚类中被逐一分开;海南万宁、广东广州和海南海口海府大道3个产地样品相似度介于0.8~0.9之间;海南文昌、广东东莞、海南乐东、海南琼海、广西南宁六安村、西双版纳勐海、西双版纳勐腊7个产地样品相似度均大于0.9,系统聚类分析能聚为一类。结论:不同产地灯台叶化学组分较相似,但含量差异明显。海南文昌、广东东莞、海南乐东、海南琼海、广西南宁六安村5个产地样品与传统药用产地西双版纳灯台叶样品质量相似,可以作为傣药灯台叶药材来源。 Objective:To establish the HPLC fingerprint of Alstonia scholaris(L.) R.Br.from different origins for identification,quality control and resource evaluation,so as to promote the development of Dai medicine modernization process.Methods:Ultrasonic extraction method was optimized by orthogonal experiment design,and the HPLC fingerprints were defined.Chromatographic conditions:The HPLC fingerprints were obtained by the Agilent ZORBAX Eclipse XDB C18(250 mm×4.6 mm,5 μm) chromatographic column with the mobile phase of water containing 0.1% formic acid(A)-acetonitrile(B) for gradient elution(0-5 min,5%B;5-35 min,5%B → 42%B;35-40 min,42%B → 80%B;40-50 min,80%B → 95%B;50-65 min,95%B) at the flow rate of 1.0 m L·min-(-1).The wavelength was set at 287 nm,the column temperature was maintained at 30 ℃,and the injection volume was 5 μL.The fingerprints of Alstonia scholaris(L.) R.Br.from different regions were analyzed by similarity evaluation and cluster analysis.Results:Ultrasonic optimum condition was 70% methyl alcohol,50 min,1∶10(ratio of solid to liquid).The HPLC fingerprint of Alstonia scholaris(L.)R.Br.was established,and totally 10 common peaks were selected.Samples from Hainan Zhonghe,Guangxi Nanning Beihu village and Hainan Haikou Gaoxinqu were separated for the similarity less than 0.8.Sample similarity of Hainan Wanning,Guangdong Guangzhou and Hainan Haikou Haifu road was between 0.8-0.9.Similarity of Hainan Wenchang,Guangdong Dongguan,Hainan Ledong,Hainan Qionghai,Guangxi Nanning Liuan village was more than 0.9,similar with Xishuangbanan Menghai and Mengla.Conclusion:Sample chemical composition from different origins is similar,but the content difference is obvious.Sample quality of Hainan Wenchang,Guangding Dongguan,Hainan Ledong,Hainan Qionghai,and Guangxi Nanning Liuan village is similar to those of traditional origin Xishuangbanan,which can be used in Dai clinic.
出处 《药物分析杂志》 CAS CSCD 北大核心 2016年第3期473-479,共7页 Chinese Journal of Pharmaceutical Analysis
基金 云南省自然科学基金重大项目(2013FC006)
关键词 灯台叶 糖胶树 傣药 指纹图谱 相似度评价 聚类分析 药材资源评价 高效液相色谱法 Folium Alstoniae Scholaris Alstonia scholaris(L.) R.Br. Dai medicine fingerprint chromatography similarity evaluation cluster analysis herbs resource evaluation HPLC
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