牛磺酸对昆明小鼠早期胚胎体外发育和着床的影响

Effectof Taurine on Development and Implantation of Kunming Mouse Early Embryos

本研究主要探讨了牛磺酸对昆明(KM)小鼠体外胚胎发育及对胚胎着床的作用。在mKSOM(modified potassium simplex optimized medium)胚胎体外培养液中分别添加不同浓度牛磺酸,培养24、72h后分别统计4-细胞率、囊胚率、囊胚总细胞数;采用子宫角注射的方法,研究牛磺酸转运抑制剂β-丙氨酸对孕D9胚胎着床数的影响。结果表明,添加10mmol/L牛磺酸培养液组的胚胎4-细胞率高于对照组(P<0.05),囊胚率与囊胚总细胞数明显高于对照组(P<0.01)。子宫角注射β-丙氨酸(20μg/mL)显著降低D9着床的胚胎数。本研究初步明确了牛磺酸对小鼠体外胚胎发育及对胚胎着床的影响,为进一步研究胚胎发育和着床提供参考。

The effects of taurine on development and implantation of Kunming mouse early embryos were investigated.The embryos were cultivated in modified potassium simplex optimized medium（mKSOM）with different concentration of taurine.The percentage of 4-cell,blastocysts and average total cell number of blastocysts were calculated after 24 hand 72h.Cornua uteri injection technique was used to investigate the effects ofβ-alaline（β-ala,taurine transport inhibitor）on embryo implantation.The results showed that there were significant differences between 10mmol/L taurine treatment and control in the percentage of 4-cell（P〈0.05）,blastocysts and total cell number of blastocysts（P〈0.01）.Meanwhile,injection ofβ-ala（20μg/mL）into uterine horn significantly reduced the number of implanted embryos on D9.This study explicated the effects of taurine on the development and implantation of early Kunming mouse embryos,providing a useful reference for further uncovering of mechanism of embryo development and implantation.