紫苏子油微囊脂肪酸甲酯化GC指纹图谱研究

Study on GC fingerprint of methyl esterification of fatty acids in Perilla oil miscrocapsule

目的:建立紫苏子油微囊脂肪酸甲酯化GC指纹图谱分析方法。方法:采用HP-5(30m×320μm,0.25μm)毛细管色谱柱;程序升温:初始温度140℃,以5℃/min升温至160℃(维持5min),然后以1℃/min升温至200℃(维持5min),再以5℃/min升温至240℃(维持10min);进样口温度250℃;FID检测器温度270℃;载气:氮气,流速0.3m L/min;进样量:1μL,分流比20∶1。采用中药色谱指纹图谱相似度评价软件(2004A版)进行相似度评价。结果:10批样品均标示出4个共有峰,鉴别了1个共有峰(亚油酸甲酯);10批样品的相似度大于0.99,提示紫苏子油微囊脂肪酸质量较稳定。结论:本方法准确可靠、重复性好,为紫苏子油微囊质量控制提供了依据。

Objective：To develop an GC method for fingerprint analysis on methyl esterification of fatty acids in Perilla oil miscrocapsule.Methods：The GC separation was performed on a HP-5 capillary column（30m ×320μm,0.25μm） and the initial temperature was programmed from 140℃ to 160℃ at 5℃·min^（-1）and kept for 5 min,then increased by 1 ℃·min^（-1） to 200 ℃,after keeping for 5 min,reincreased by 5 ℃·min^（-1）to 240℃ and maintain for 10 min. Inject temperature at 250℃,and the FID detector temperature was at 270℃. Carrier gas was N2, and the folw rate was 0.3m L·min^（-1）with the split ratio of 20 ∶1.The similarity was evaluated on the Similarity evaluation system for chromatographic fingerprint of Traditional Chinese Medicine. Results：4 common peaks were separated from 10 batches of Perilla oil miscrocapsule and one of them（methyl glyoxalidine linoleate）has been identified. The similarities of 10 batches were above 0.99, which indicated the quality of Perilla oil miscrocapsule was relatively stable. Conclusion：The method is accurate and reproducible, which can be used for the quality control of Perilla oil miscrocapsule.