酵母菌还原制备纳米金颗粒

Biological Synthesis of Gold Nanoparticles by Saccharomyces

纳米金颗粒因其独特的光学、电学和催化性质而被广泛应用于生物传感器、肿瘤治疗、重金属分析、化工催化等领域。生物制备方法具有良好的生物相容性、反应条件温和、绿色且可持续发展等优点。本文利用酵母菌制备了纳米金颗粒,采用UV-Vis研究了反应条件,FESEM、EDS及TEM表征了材料的形貌和成分,并通过FT-IR探究酵母菌还原金颗粒的机制。结果表明,当反应时间为60 min、贵金属前驱体浓度为1.0 g/L时,酵母菌还原所得的金纳米颗粒粒径约为8.69 nm且大小比较均一。红外分析结果表明酵母菌在还原贵金属前驱体时,多羟基化合物、蛋白质类物质等起还原作用。

Gold nanoparticles have been widely applied in many fields, such as biological sensors, tumourtreatments, analysis of heavy metals and catalytic reactions, due to their unique optional, electronic andcatalytic properties. The biological synthesis method has many advantages, such as biocompatibility ofproducts, mild reaction conditions, more green and sustainable. Gold nanoparticles were prepared bysaccharomyces in this paper. The UV-Vis was used to study the reaction conditions, FESEM, EDS andTEM have been employed to determine the shape and content of gold nanoparticles. FT-IR was used toanalysis the change of saccharomyces before and after reduction. It shows that when the reaction time was60 min, the concentration of precursor was 1.0 g/L, the obtained gold particles were uniform, and theirmean size was 8.69 nm. It is inferred that multiple-hydroxyl compound, proteins were involved in thereduction reaction from FT-IR results.