雷公藤多苷配伍黄芪总皂苷对大鼠关节炎的减毒增效作用

Toxicity-reducing and Action-enhancing Effects of Tripterginum Wilfordii Polyglycoside Combined with Astragalosides on Rat Adjuvant Arthritis

目的:研究雷公藤多苷(tripterginum wilfordii polyglycoside,TWP)配伍黄芪总皂苷(astragalosides,ASTs)对佐剂性关节炎(AA)大鼠的治疗效果及肝肾功能的影响。方法:弗式完全佐剂建立AA大鼠模型,造模后第1d各组给药,连续28d,测量大鼠原发性和继发性足肿胀程度,检测血清中白介素-1β(IL-1β)和丙二醛(MDA)水平,检测大鼠的肝肾功能。结果:(1)相较于AA模型组,雷公藤多苷组(雷公藤多苷10mgkg-1,1次/d)、黄芪总皂苷组(黄芪总皂苷100mgkg-1,1次/d)和配伍组(雷公藤多苷10mgkg-1+黄芪总皂苷100mgkg-1,1次/d)中大鼠足爪肿胀程度均显著下降(P<0.01),其中以配伍组大鼠关节炎症状改善最为明显;(2)模型组大鼠血清IL-1β水平明显升高,而经过治疗后血清中IL-1β含量下降,且以配伍组降低最为显著;(3)与模型组相比,雷公藤多苷组大鼠的谷草转氨酶(AST)、谷丙转氨酶(ALT)、丙二醛(MDA)、血尿素氮(BUN)和肌酐(Cr)均显著增高(P<0.01或P<0.05);(4)与雷公藤多苷组相比,配伍组大鼠的AST、ALT、MDA、BUN和Cr均显著降低。结论:雷公藤多苷配伍黄芪总皂苷使用可显著降低血清中炎症因子水平,减轻AA大鼠的关节炎症状,并具有减毒增效的作用。

Objective:To study the therapeutic effects and possible mechanism of tripterginum wilfordii polyglycoside(TWP)combined with astragalosides (ASTs)on rat adj uvant arthritis (AA).Methods:The murine collagen-induced arthritis model was established,after treatment,the rats paw edema were evaluated, the level of serum interleukin-1 beta(IL-1β)and malondialdehyde (MDA)was detected,and the index of liver and kidney function were detected by some kit .Results:(1 )Compared with AA model group,the hind paw swelling in TWP group,ASTs group and TWP+ ASTs group were significantly decreased (P<0.01),espe-cially in TWP+ ASTs group (P<0.05);(2)The levels of serum IL-1βwas notably higher in AA model group than control group (P<0.01),which was significantly decreased in three treatment groups(P<0.01 or P<0.05);(3)Compared with AA model group,the levels of AST、ALT、MDA、BUN and SCr in TWP group were significantly decreased(P<0.01or P<0.05);(4)Compared with TWP group,the levels of AST、ALT、MDA、BUN and SCr in TWP+ ASTs group were significantly decreased (P<0.01).Conclusion:The combined use of TWP and ASTs can significantly relieve the arthritis syndrome in AA rats by reducing the expression of serum cytokines,and its therapeutic effect and the effect of toxicity-reducing is better than used separately.