BSP技术在萝卜-芥蓝异源四倍体及其亲本BZIP17同源基因甲基化检测中的应用

Application of BSP Method to the Methylation Detection of BZIP17 Homologous Genes in Allotetraploid Raphanobrassica and Its Parents

为探讨异源多倍体基因组中直系同源基因的表达调控机制，对重亚硫酸盐测序PCR（BSP）技术进行了改进优化。结果表明，改进的BSP技术检测到萝卜-芥蓝四倍体及其亲本BZIPl7同源基因启动子的甲基化水平为3．8％～18．8％，采用实时荧光定量PCR检测BZIPl7基因的相对表达量，且BZIPl7同源基因的表达调控与启动子甲基化等作用相关。因此，改进的BSP技术可应用到更多同源基因的甲基化检测中，以分析异源多倍体中同源基因的分子进化方式。

In order to understand the expression regulation mechanism of parent origin orthologous genes in the allopolyploid genome, the bisulfite sequencing PCR(BSP) method was optimized. The results showed that the methylaiton rate of BZIP17 homologous gene promoters in allotetraploid Raphanobrassica and its parents was ranged from 3.8% to 18.8%. Moreover, the expression level of BZIP17 genes in the allotetraploid and its parents were detected using quantitative real-time PCR, which summarized that the expression regulation of BZIP17 homologous genes is correlated with promoter methylation and other mechanisms. So, it was suggested that the BSP method could be applied to the methylation detection of more homologous genes, for the researches of molecular evolution patterns of allopolyploid homologous genes.