2013—2014年云南省剑川县野鼠鼠疫疫源地小肠结肠炎耶尔森菌的调查分析

Investigation of Yersinia enterocolitica in wild plague foci in Jianchuan,Yunnan

目的对剑川县野鼠鼠疫疫源地小肠结肠炎耶尔森菌分布情况调查分析。方法从剑川采集鼠盲结肠标本进行小肠结肠炎耶尔森菌分离培养、表型鉴定和特异基因分析。结果从采集的645份鼠类标本中,分离到34株小肠结肠炎耶尔森菌,分离率为5.27%(34/645);其中致病株1株、非致病株33株。鼠类标本分别是大绒鼠、贝氏树鼩、褐家鼠、齐氏姬鼠、小林姬鼠等,其中大绒鼠采集率和检出率最高(2.79%),其次为齐氏姬鼠(1.55%)。结论剑川县野鼠鼠疫疫源地中,小肠结肠炎耶尔森菌的主要宿主与鼠疫菌主要宿主基本一致,推测小肠结肠炎耶尔森菌的存在与鼠疫的相对静息相关。

Objective To investigate the distribution of Yersinia enterocolitica in the foci of wild plague in Jianchuan county,Yunnan province. Methods The caecum and colon specimens of wild rats from Jianchuan were collected and then Y. enterocolitica were isolated and cultured. Results A total of 34 Y. enterocolitica strains,including 1 pathogenic strain and33 non-pathogenic or weak pathogenic strains,were isolated,the overall positive rate was 5. 27%（ 34 /645）. The rodent samples included Eothenomys miletus app： addword： Yunnan red-backed vole,bayesian tree shrews,brown rats,Apodemus chevrieri and Apodemus syhaticus Linnaeus,etc. The detection rate of Y. enterocolitica in Eothenomys miletus was highest（ 2. 79%）,followed by that in bayesian tree shrews（ 1. 55%）. Conclusion The main hosts of Y. enterocolitica and the main hosts of Yersinia pestis were same in Jianchuan,suggesting that the presence of Y. enterocolitica might be related with silence of plague.