建立鼻咽癌标本库的经验和体会

Experiences from establishing a nasopharyngeal carcinoma specimen bank

目的利用我院鼻咽癌的标本资源,建立鼻咽癌标本库,为科学研究提供组织标本。方法收集鼻咽癌患者的组织标本及外周血液。将组织标本置于-196℃液氮中保存;将分别处理后的全血液、白细胞层、血浆和血清于-80℃低温冰箱长期保存。将所有资料数据信息录入计算机系统。结果目前标本库保存的鼻咽癌组织标本共1 152例,其中原发恶性肿瘤1 151例,复发肿瘤标本1例。鼻咽部正常组织标本95例。鼻咽慢性炎症标本24例。全血液标本、血浆标本、白细胞层标本和血清标本各2 416例。血液标本治疗前1 635例,治疗结束781例,治疗前和治疗结束配对499例。血液标本和组织标本配对614例,其中与治疗前、治疗结束的血液标本均配对者193例。结论初步建立了具有一定规模的鼻咽癌标本库,为标本库的发展积累了一定的经验。

Objective To describe the process and experiences of setting up a specimen bank of nasopharyngeal cancer （NPC） tumor tissue at Fujian Provincial Tumor Hospital as a resource for clinical and basic research. Methods Tissue and blood samples were collected from NPC patients and transported to the laboratory in a liquid nitrogen container and stored at -196 ℃. Whole blood and separate fractions of plasma,serum,and buffy coat were prepared and stored at -80 ℃. Clinical and demographic data for patient tissue donors were recorded. Results The specimen bank contains 1,152 primary NPC tissue specimens,of which 1,151 are from primary malignant tumor while 1 is from a recurrent tumor;95 nasopharyngeal tissue samples from healthy control individuals;24 tissue samples from individuals with chronic NPC;and 2,416 samples of blood,plasma, buffy coat,and serum. Of the 2,416 blood samples,1,635 were collected before treatment and 781 were collected after treatment,making 499 matched pairs of pre-/post-treatment samples. There are 614 matched pairs of blood samples and tissues, among which there are 193 matched pairs of pre-/post-treatment blood samples. Conclusion An NPC specimen bank has been established at Fujian Provincial Tumor Hospital,and it is ready to provide material for research as well as to continue growing.