蔗糖处理对费菜黄酮含量及其抗氧化性的影响

Effect of Sucrose Treatment on Flavonoid Content and Antioxidant Activity of Sedum aizoon Leaves

本文以费菜为研究对象,采用不同浓度5、10、20、30、40 mmol/L蔗糖培养液对费菜嫩枝进行培养,选取费菜黄酮含量富集作用较强的蔗糖培养液,并研究该浓度下费菜的抗氧化性。结果表明:选取20 mmol/L蔗糖培养液对费菜培养,对黄酮有较好的富集作用,该浓度培养液的费菜黄酮含量(鲜重)平均值为对照组的1.58倍;蔗糖处理明显提升了费菜DPPH自由基清除能力及总酚的含量;与对照组相比,蔗糖处理组苯丙氨酸解氨酶(phenylalanine ammonialyas,PAL)酶活力也一直保持较高水平;超氧化物歧化酶(superoxide dismutase,SOD)、抗坏血酸过氧化物酶(ascorbate peroxidase,APX)、过氧化物酶(peroxidase,POD)等抗氧化酶活力在外源蔗糖的影响下均高于对照组。这些结果表明了外源蔗糖处理较好地提高了费菜黄酮含量和抗氧化性,为费菜黄酮的富集提供了新的思路和理论依据。

Young twigs from Sedum aizoon L. were incubated in media containing various concentrations of sucrose（5, 10, 20, 30, and 40 mmol/L）. The medium containing 20 mmol/L sucrose exhibited a relatively good flavonoid accumulation during the incubation, with the flavonoid content（wet weight） of Sedum aizoon L. cultured in this medium being 1.58 times greater than that of the control group. This medium was selected to study the antioxidant capacity of Sedum aizoon L. Sucrose treatment significantly increased the 2,2-diphenyl-1-picrylhydrazyl（DPPH） radical-scavenging activity and total phenolic content. The sucrose-treated group had higher activities of superoxide dismutase（SOD）, ascorbate peroxidase（APX）, and peroxidase（POD） than the control group due to the impact of exogenous sucrose. In addition, the phenylalanine ammonia-lyase（PAL） activity for the sucrose-treated group was higher than that of the control group. These results indicate that sucrose treatment enhances flavonoid content and antioxidant activity. These results provide a new approach and a theoretical basis for flavonoid accumulation using Sedum aizoon L.