基于氧化石墨烯荧光适体传感器的胰岛素检测

An Aptamer-based Fluorescence Biosensor for Insulin Detection Based on Graphene Oxide

采用荧光基团(FAM)标记的核酸适体作为识别元件,氧化石墨烯为淬灭剂,建立了一种高选择性、高灵敏度的核酸适体传感器。核酸适体与氧化石墨烯结合后,荧光淬灭,此时溶液无荧光;加入胰岛素后,溶液中荧光得到恢复。利用荧光分析法检测加入胰岛素前后,溶液中荧光强度的变化,获取了荧光适体传感器的线性度和灵敏度,实现对胰岛素浓度的测定。结果表明,在5×10^(-8)~1×10^(-5)mol/L范围内,胰岛素的浓度与溶液中荧光强度有良好的线性关系,检出限为10 nmol/L。采用此方法检测胰岛素,操作简便,检测速度快,准确性高,选择性好,检出限低。

By using fluorophore （FAM） labeled aptamers as recognition elements and graphene oxide as a quencher, a highly selective and sensitive sensor was constructed for the fast and accurate detection of insulin. After the aptamer binding with graphene oxide, fluorescence will be quenched and fluorescence intensity disappeared; after addition of insulin, the solution fluorescence was restored. Based on the fact, we established a method for the determination of insulin concentration by measuring the fluorescence intensity. The results showed that the concentration of insulin in the range of 5× 104 - 1 × 10-5 mol/L had a good linear relationship with the fluorescence intensity. The detection limit was 10 nmol/L. This method of detecting insulin had obvious advantages of fast detection speed, high selectivity and low detection limit.