摘要
[目的]应用荧光光度法检测戊唑醇。[方法]以牛血清白蛋白作荧光探针,利用戊唑醇对牛血清白蛋白的内源荧光猝灭作用,建立荧光光度法检测戊唑醇。[结果]在优化条件下,荧光强度变化与戊唑醇浓度在8~102μg/ml范围内呈线性关系,相关系数为O.9932,检出限为0.66μg/ml。对戊唑醇模拟水样进行标准加入回收率试验,回收率为95.6%-104.1%,相对标准偏差均低于4.1%。[结论]建立的检测方法较简单。
[Objective]The aim of the study was to detect tebuconazole using fluorescence spectrophotometry. [Method] Based .on the intrinsic fluorescence intensity of BSA as the probe was quenched in proportional to the concen- 'tration of tebuconazole, a fluorescence spectrophotometric method for the determination of tebuconazole was established. [Result] Under optimal condi- tions, a linear relationship between the fluorescence intensity change and the tebuconazole concentration in the range from 8 to 102 μg/ml with the correlation coefficient of 0.993 2. The detection limit was 0,66 μg/ml. The method has been applied for the determination of tebuconazole simulated water with the recovery from 95.6% to 104.1% and the RSD lower than 4.1%. [Conlusion] The method was simple and sensitive.
出处
《农业灾害研究》
2016年第1期5-7,共3页
Journal of Agricultural Catastrophology
基金
2013年全国大学生创新训练项目"三唑类农药残留的检测"(201310363)
关键词
戊唑醇
牛血清白蛋白
荧光光度法
Tebuconazole
Bovinealbumin
Fluorescence spec-trophotometry