荧光光度法测定戊唑醇的研究

Fluorescence Spectrop hot-ometric Method for the Determination of Tebu-conazole

[目的]应用荧光光度法检测戊唑醇。[方法]以牛血清白蛋白作荧光探针，利用戊唑醇对牛血清白蛋白的内源荧光猝灭作用，建立荧光光度法检测戊唑醇。[结果]在优化条件下，荧光强度变化与戊唑醇浓度在8～102μg／ml范围内呈线性关系，相关系数为O．9932，检出限为0．66μg／ml。对戊唑醇模拟水样进行标准加入回收率试验，回收率为95．6％-104．1％，相对标准偏差均低于4．1％。[结论]建立的检测方法较简单。

[Objective]The aim of the study was to detect tebuconazole using fluorescence spectrophotometry. [Method] Based .on the intrinsic fluorescence intensity of BSA as the probe was quenched in proportional to the concen- ＇tration of tebuconazole, a fluorescence spectrophotometric method for the determination of tebuconazole was established. [Result] Under optimal condi- tions, a linear relationship between the fluorescence intensity change and the tebuconazole concentration in the range from 8 to 102 μg/ml with the correlation coefficient of 0.993 2. The detection limit was 0,66 μg/ml. The method has been applied for the determination of tebuconazole simulated water with the recovery from 95.6% to 104.1% and the RSD lower than 4.1%. [Conlusion] The method was simple and sensitive.