has-miR-16靶基因预测及生物信息学分析

Prediction of has-miR-16 target genes and its bioinformatics analysis

目的预测has-miR-16靶基因并对靶基因的分子功能、生物学过程和信号转导通路富集性分析,全面认识miR-16涉及的生命过程和疾病。方法通过阅读文献结合生物信息学预测的方法得出靶基因集合,用Cytoscape 3.2.1软件和DAVID(the database for annnotation visualization and integrated discovery)数据库分别对靶基因GO(gene ontology)注释和KEGG(kyoto encyclopedia of genes and genomes)信号转导通路进行富集性分析。结果各物种已知的成熟miR-16序列具有高度保守性。397个靶基因在分子功能上显著富集于蛋白结合、DNA结合、酶类活性、胰岛素样生长因子受体活性等功能,在生物学过程显著富集于发育过程、细胞粘附、神经发生、细胞代谢和细胞周期调控等过程。靶基因显著富集的信号通路有癌症通路、p53信号通路、神经营养因子信号通路等通路。结论从分析结果得出has-miR-16通过调控靶基因参与了人类生命活动和疾病过程的很多方面,尤其在癌症的病理过程是一个值得进一步研究的方向。

Objective The aim is to predict the target genes of has-miR-16 and to analyze the molecular function,biological process and signal pathway enrichment of target genes,and comprehensively know living activity process and disease associated with miR-16.Methods The collection of target genes were predicted with bioinformatics methods and relevant literature.Cytoscape 3.2.1 and the database for annnotation visualization and integrated discovery（ DAVID） database were used to analyze the GO（ gene ontology） annotation and KEGG（ kyoto encyclopedia of genes and genomes） signal pathway enrichment respectively.Results The mature sequence of miR-16 among species had highly conservative property.The molecular functions of 397 target genes were significantly invovled in protein binding,DNA binding,enzyme activity,and insulin-like growth factor receptor activity,etc.Biological processes of these genes were significantly associated with the process of development,cell adhesion,neurogenesis,cell metabolism and cell cycle regulation process.These target genes were also involved in cancer pathway,p53 signal pathway,neurotrophin signal pathway.Conclusions Has-miR-16 is involve in some human living activities and disease process through regulating target genes,especially cancer.