撒坝猪成纤维细胞系的建立及其生物学特性分析

Establishment and the Biological Characteristics of Fibroblast Cell Line in Saba Pig

实验采用撒坝猪耳缘组织块贴壁培养法首次对撒坝猪成纤维细胞进行体外培养,通过原代培养、传代培养、冷冻保存建立了撒坝猪耳缘成纤维细胞系;并对其细胞形态、细胞活率、细胞核型等生物学特性进行分析;还利用透射电镜观察了耳缘组织细胞。结果表明:细胞倍增时间为48 h,生长曲线为"S"型;染色体中正常二倍体染色体(2n=38)所占比例为92.56%;细菌、真菌、病毒和支原体等微生物污染检测均显示为阴性。透射电镜观察显示,成纤维细胞呈长梭型。

In this study, the fibroblasts cell line derived from ear marginal tissue of saba pig was successfully established using the primary explants technique and cryopreservation technology. The morphology,cell survival rate and karyotypes of the cultured cells after different passages were analyzed,The tissue cells of the ear were observed by transmission electron microscopy. The results showed that the population doubling time（ PDT） of the cells was 48 h; The growth curve of the cultured cells appeared＂S＂ shape; The frequency of cell chromosome number to be 2n = 38 was 92. 56 %; Tests for the contamination from bacteria, fungi or mycoplasma were negative; Transmission electron microscopy showed that fibroblast cells were long shuttle type.